@article{Tunstall:2017:10.​1016/​j.​ebiom.​2017.​03.​033,
author = {Tunstall, T and Kon, OM and Bartlett, N and Hansel, TT and Johnston, SL and Mallia, P and Jackson, DJ and Walton, R and Edwards, M and Trujillo-Torralbo, MB and del-Rosario, A and Shamji, B and Dhariwal, J and Kirk, P and Stumpf, M and Koopmann, JO and Telcian, A and Aniscenko, J and Gogsadze, L and Bakhsoliani, E and Stanciu, L and Hunt, TM and Hunt, TL and Hunt, DG and Westwick, J and Edwards, M},
doi = {10.1016/j.ebiom.2017.03.033},
journal = {EBioMedicine},
pages = {128--138},
title = {A Comprehensive Evaluation of Nasal and Bronchial Cytokines and Chemokines Following Experimental Rhinovirus Infection in Allergic Asthma: Increased Interferons (IFN-γ and IFN-λ) and Type 2 Inflammation (IL-5 and IL-13)},
url = {http://hdl.handle.net/10044/1/45996},
volume = {19},
year = {2017}
}

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TY  - JOUR
AB  - BackgroundRhinovirus infection is a major cause of asthma exacerbations.ObjectivesWe studied nasal and bronchial mucosal inflammatory responses during experimental rhinovirus-induced asthma exacerbations.MethodsWe used nasosorption on days 0, 2–5 and 7 and bronchosorption at baseline and day 4 to sample mucosal lining fluid to investigate airway mucosal responses to rhinovirus infection in patients with allergic asthma (n = 28) and healthy non-atopic controls (n = 11), by using a synthetic absorptive matrix and measuring levels of 34 cytokines and chemokines using a sensitive multiplex assay.ResultsFollowing rhinovirus infection asthmatics developed more upper and lower respiratory symptoms and lower peak expiratory flows compared to controls (all P < 0.05). Asthmatics also developed higher nasal lining fluid levels of an anti-viral pathway (including IFN-γ, IFN-λ/IL-29, CXCL11/ITAC, CXCL10/IP10 and IL-15) and a type 2 inflammatory pathway (IL-4, IL-5, IL-13, CCL17/TARC, CCL11/eotaxin, CCL26/eotaxin-3) (area under curve day 0–7, all P < 0.05). Nasal IL-5 and IL-13 were higher in asthmatics at day 0 (P < 0.01) and levels increased by days 3 and 4 (P < 0.01). A hierarchical correlation matrix of 24 nasal lining fluid cytokine and chemokine levels over 7 days demonstrated expression of distinct interferon-related and type 2 pathways in asthmatics. In asthmatics IFN-γ, CXCL10/IP10, CXCL11/ITAC, IL-15 and IL-5 increased in bronchial lining fluid following viral infection (all P < 0.05).ConclusionsPrecision sampling of mucosal lining fluid identifies robust interferon and type 2 responses in the upper and lower airways of asthmatics during an asthma exacerbation. Nasosorption and bronchosorption have potential to define asthma endotypes in stable disease and at exacerbation.
AU  - Tunstall,T
AU  - Kon,OM
AU  - Bartlett,N
AU  - Hansel,TT
AU  - Johnston,SL
AU  - Mallia,P
AU  - Jackson,DJ
AU  - Walton,R
AU  - Edwards,M
AU  - Trujillo-Torralbo,MB
AU  - del-Rosario,A
AU  - Shamji,B
AU  - Dhariwal,J
AU  - Kirk,P
AU  - Stumpf,M
AU  - Koopmann,JO
AU  - Telcian,A
AU  - Aniscenko,J
AU  - Gogsadze,L
AU  - Bakhsoliani,E
AU  - Stanciu,L
AU  - Hunt,TM
AU  - Hunt,TL
AU  - Hunt,DG
AU  - Westwick,J
AU  - Edwards,M
DO  - 10.1016/j.ebiom.2017.03.033
EP  - 138
PY  - 2017///
SN  - 2352-3964
SP  - 128
TI  - A Comprehensive Evaluation of Nasal and Bronchial Cytokines and Chemokines Following Experimental Rhinovirus Infection in Allergic Asthma: Increased Interferons (IFN-γ and IFN-λ) and Type 2 Inflammation (IL-5 and IL-13)
T2  - EBioMedicine
UR  - http://hdl.handle.net/10044/1/45996
VL  - 19
ER  - 

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