Publications
40 results found
Barr AR, Bakal C, 2015, A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly (vol 5, 10564, 2015), SCIENTIFIC REPORTS, Vol: 5, ISSN: 2045-2322
Barr AR, Bakal C, 2015, A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly, Scientific Reports, Vol: 5, ISSN: 2045-2322
How multiple spindle assembly pathways are integrated to drive bipolar spindle assembly is poorly understood. We performed an image-based double RNAi screen to identify genes encoding Microtubule-Associated Proteins (MAPs) that interact with the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells. We identified a ch-TOG centred network of genetic interactions which promotes centrosome-mediated microtubule polymerisation, leading to the incorporation of microtubules polymerised by all pathways into a bipolar structure. Our genetic screen also reveals that ch-TOG maintains a dynamic microtubule population, in part, through modulating HSET activity. ch-TOG ensures that spindle assembly is robust to perturbation but sufficiently dynamic such that spindles can explore a diverse shape space in search of structures that can align chromosomes.
Yin Z, Sadok A, Sailem H, et al., 2013, A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes, NATURE CELL BIOLOGY, Vol: 15, Pages: 860-+, ISSN: 1465-7392
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- Citations: 130
Barr AR, Bakal C, 2012, A direct look at RNAi screens, Molecular Systems Biology, Vol: 8, ISSN: 1744-4292
Sir J-H, Barr AR, Nicholas AK, et al., 2011, A primary microcephaly protein complex forms a ring around parental centrioles, NATURE GENETICS, Vol: 43, Pages: 1147-U150, ISSN: 1061-4036
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- Citations: 160
Barr AR, Kilmartin JV, Gergely F, 2010, CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response, Journal of Cell Biology, Vol: 189, Pages: 23-39, ISSN: 0021-9525
The centrosomal protein, CDK5RAP2, is mutated in primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size. The Drosophila melanogaster homologue of CDK5RAP2, centrosomin (Cnn), maintains the pericentriolar matrix (PCM) around centrioles during mitosis. In this study, we demonstrate a similar role for CDK5RAP2 in vertebrate cells. By disrupting two evolutionarily conserved domains of CDK5RAP2, CNN1 and CNN2, in the avian B cell line DT40, we find that both domains are essential for linking centrosomes to mitotic spindle poles. Although structurally intact, centrosomes lacking the CNN1 domain fail to recruit specific PCM components that mediate attachment to spindle poles. Furthermore, we show that the CNN1 domain enforces cohesion between parental centrioles during interphase and promotes efficient DNA damage–induced G2 cell cycle arrest. Because mitotic spindle positioning, asymmetric centrosome inheritance, and DNA damage signaling have all been implicated in cell fate determination during neurogenesis, our findings provide novel insight into how impaired CDK5RAP2 function could cause premature depletion of neural stem cells and thereby microcephaly.
Barr AR, Zyss D, Gergely F, 2009, Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways, MITOSIS: METHODS AND PROTOCOLS, Vol: 545, Pages: 1-19, ISSN: 1064-3745
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- Citations: 2
Barr AR, Gergely F, 2008, MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics, MOLECULAR AND CELLULAR BIOLOGY, Vol: 28, Pages: 7199-7211, ISSN: 0270-7306
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- Citations: 41
Barr AR, Gergely F, 2007, Aurora-A: the maker and breaker of spindle poles, JOURNAL OF CELL SCIENCE, Vol: 120, Pages: 2987-2996, ISSN: 0021-9533
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- Citations: 347
Osman F, Dixon J, Barr AR, et al., 2005, The F-box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins, MOLECULAR AND CELLULAR BIOLOGY, Vol: 25, Pages: 8084-8096, ISSN: 0270-7306
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- Citations: 100
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