Imperial College London
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DrAlexisBarr

Faculty of Medicine, Institute of Clinical Sciences

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 7772a.barr Website

 
 
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Location

 

Office 6.12BLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stojic:2020:10.1038/s41467-020-14978-7,
author = {Stojic, L and Lun, ATL and Mascalchi, P and Ernst, C and Redmond, AM and Mangei, J and Barr, AR and Bousgouni, V and Bakal, C and Marioni, JC and Odom, DT and Gergely, F},
doi = {10.1038/s41467-020-14978-7},
journal = {Nature Communications},
pages = {1--21},
title = {A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division},
url = {http://dx.doi.org/10.1038/s41467-020-14978-7},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs, we identify numerous lncRNAs involved in key steps of cell division such as chromosome segregation, mitotic duration and cytokinesis. Here, we provide evidence that the chromatin-associated lncRNA, linc00899, leads to robust mitotic delay upon its depletion in multiple cell types. We perform transcriptome analysis of linc00899-depleted cells and identify the neuronal microtubule-binding protein, TPPP/p25, as a target of linc00899. We further show that linc00899 binds TPPP/p25 and suppresses its transcription. In cells depleted of linc00899, upregulation of TPPP/p25 alters microtubule dynamics and delays mitosis. Overall, our comprehensive screen uncovers several lncRNAs involved in genome stability and reveals a lncRNA that controls microtubule behaviour with functional implications beyond cell division.
AU  - Stojic,L
AU  - Lun,ATL
AU  - Mascalchi,P
AU  - Ernst,C
AU  - Redmond,AM
AU  - Mangei,J
AU  - Barr,AR
AU  - Bousgouni,V
AU  - Bakal,C
AU  - Marioni,JC
AU  - Odom,DT
AU  - Gergely,F
DO  - 10.1038/s41467-020-14978-7
EP  - 21
PY  - 2020///
SN  - 2041-1723
SP  - 1
TI  - A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-14978-7
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000564285200016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41467-020-14978-7
UR  - http://hdl.handle.net/10044/1/84623
VL  - 11
ER  -
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