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BibTex format

@article{Hegarat:2020:10.15252/embj.2020104419,
author = {Hegarat, N and Crncec, A and Rodriguez, MFSP and Iturra, FE and Gu, Y and Busby, O and Lang, PF and Barr, AR and Bakal, C and Kanemaki, MT and Lamond, A and Novak, B and Ly, T and Hochegger, H},
doi = {10.15252/embj.2020104419},
journal = {The EMBO Journal},
pages = {1--23},
title = {Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B},
url = {http://dx.doi.org/10.15252/embj.2020104419},
volume = {39},
year = {2020}
}

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TY  - JOUR
AB  - Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects mitosis. Loss of cyclin A in G2phase prevents mitotic entry. Cells lacking cyclin B can enter mitosis and phosphorylate most mitotic proteins, because of parallel PP2A:B55 phosphatase inactivation by Greatwall kinase. The final barrier to mitotic establishment corresponds to nuclear envelope breakdown, which requires a decisive shift in the balance of cyclindependent kinase Cdk1 and PP2A:B55 activity. Beyond this point, cyclin B/Cdk1 is essential for phosphorylation of a distinct subset of mitotic Cdk1 substrates that are essential to complete cell division. Our results identify how cyclin A, cyclin B and Greatwall kinase coordinate mitotic progression by increasing levels of Cdk1dependent substrate phosphorylation.
AU  - Hegarat,N
AU  - Crncec,A
AU  - Rodriguez,MFSP
AU  - Iturra,FE
AU  - Gu,Y
AU  - Busby,O
AU  - Lang,PF
AU  - Barr,AR
AU  - Bakal,C
AU  - Kanemaki,MT
AU  - Lamond,A
AU  - Novak,B
AU  - Ly,T
AU  - Hochegger,H
DO  - 10.15252/embj.2020104419
EP  - 23
PY  - 2020///
SN  - 0261-4189
SP  - 1
TI  - Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B
T2  - The EMBO Journal
UR  - http://dx.doi.org/10.15252/embj.2020104419
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000539233000006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.embopress.org/doi/full/10.15252/embj.2020104419
UR  - http://hdl.handle.net/10044/1/84624
VL  - 39
ER  -

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