Imperial College London
Alternate

DrAndrewBlagborough

Faculty of Natural Sciences, Department of Life Sciences

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5350a.blagborough

 
 
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Location

 

603Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Iyori:2017:10.1111/pim.12498,
author = {Iyori, M and Blagborough, AM and Sala, KA and Nishiura, H and Takagi, K and Yoshida, S},
doi = {10.1111/pim.12498},
journal = {Parasite Immunology},
title = {Protective efficacy of an IL-12-expressing baculoviral malaria vaccine},
url = {http://dx.doi.org/10.1111/pim.12498},
volume = {39},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Interleukin-12 (IL-12) plays an important role in antigen-specific adaptive immunity against Plasmodium sporozoites, and this requirement allows for a new approach to developing an effective malaria vaccine. In this study, we examined whether IL-12 could enhance protective efficacy of a baculovirus-based malaria vaccine. For this aim, a baculoviral vector expressing murine IL-12 (mIL-12) under the control of CMV promoter (BES-mIL-12-Spider) and a baculoviral vector expressing Plasmodium falciparum circumsporozoite protein (PfCSP) with post-transcriptional regulatory element of woodchuck hepatitis virus (BDES-sPfCSP2-WPRE-Spider) were generated. BES-mIL-12-Spider produced bioactive IL-12 which activates splenocytes, resulting in induction of IFN-γ. When co-immunized with BES-mIL-12-Spider and BDES-sPfCSP2-WPRE-Spider, the mouse number for high IgG2a/IgG1 ratios and the geometric mean in this group were both increased as compared with those of the other groups, indicating a shift towards a Th1-type response following immunization with BES-mIL-12-Spider. Finally, immunization with BDES-sPfCSP2-WPRE-Spider plus BES-mIL-12-Spider had a higher protective efficacy (73%) than immunization with BDES-sPfCSP2-WPRE-Spider alone (30%) against challenge with transgenic Plasmodium berghei sporozoites expressing PfCSP. These results suggest that co-administration of IL-12 expressing baculoviral vector, instead of IL-12 cDNA, with viral-vectored vaccines provides a new feasible vaccine platform to enhance Th1-type cellular immune responses against Plasmodium parasites.
AU  - Iyori,M
AU  - Blagborough,AM
AU  - Sala,KA
AU  - Nishiura,H
AU  - Takagi,K
AU  - Yoshida,S
DO  - 10.1111/pim.12498
PY  - 2017///
SN  - 0141-9838
TI  - Protective efficacy of an IL-12-expressing baculoviral malaria vaccine
T2  - Parasite Immunology
UR  - http://dx.doi.org/10.1111/pim.12498
UR  - http://hdl.handle.net/10044/1/56186
VL  - 39
ER  -
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