Imperial College London

DrAndreasBruckbauer

Faculty of MedicineNational Heart & Lung Institute

FILM Manager
 
 
 
//

Contact

 

+44 (0)20 7594 2023a.bruckbauer Website

 
 
//

Location

 

open desk space desk 401Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Summary

Andreas Bruckbauer is Manager of the FILM Microscopy Facility at Imperial College. He joined Imperial in 2016 after working as Microscopist Developer with Facundo Batista at the Francis Crick Institute / CRUK London Research Institute. Prior to this he completed his postdoctoral training in the laboratory of Professor David Klenerman in the Chemistry Department of Cambridge University (UK) after graduating in Physics and receiving his PhD from the University of Düsseldorf.

He has successfully implemented the super-resolution microscopy techniques Direct Stochastic Reconstruction Microscopy (dSTORM) and Structured Illumination Microscopy (SIM) for research in Immunology. This allowed the publication of the first super-resolution images of endogenous B cell receptors, showing that these receptors are pre-clustered before B-cell activation. He improved the depth penetration for two-photon microscopy in lymph nodes utilising this technique for imaging of iNKT cells. His research at Cambridge University was focussed on implementing Single Molecule Tracking in Total Internal Fluorescence Microscopy (TIRFM) in combination with the deposition of biomolecules from a nanopipette using Scanning Ion-conductance Microscopy (SICM).

 

Selected Publications

Journal Articles

Gaya M, Barral P, Burbage M, et al., 2017, Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells., Cell, Vol:172, ISSN:0092-8674, Pages:517-533.e20

Martinez-Martin N, Maldonado P, Gasparrini F, et al., 2017, A switch from canonical to noncanonical autophagy shapes B cell responses, Science, Vol:355, ISSN:0036-8075, Pages:641-647

Gaya M, Castello A, Montaner B, et al., 2015, Inflammation-induced disruption of SCS macrophages impairs B cell responses to secondary infection, Science, Vol:347, ISSN:0036-8075, Pages:667-672

Mattila PK, Feest C, Depoil D, et al., 2013, The actin and tetraspanin networks organize receptor nanoclusters to regulate B cell receptor-mediated signaling., Immunity, Vol:38, Pages:461-474

Thaunat O, Granja AG, Barral P, et al., 2012, Asymmetric Segregation of Polarized Antigen on B Cell Division Shapes Presentation Capacity, Science, Vol:335, ISSN:0036-8075, Pages:475-479

More Publications