Andreas Bruckbauer is Manager of the FILM Microscopy Facility at Imperial College. He joined Imperial in 2016 after working as Microscopist Developer with Facundo Batista at the Francis Crick Institute / CRUK London Research Institute. Prior to this he completed his postdoctoral training in the laboratory of Professor David Klenerman in the Chemistry Department of Cambridge University (UK) after graduating in Physics and receiving his PhD from the University of Düsseldorf.
He has successfully implemented the super-resolution microscopy techniques Direct Stochastic Reconstruction Microscopy (dSTORM) and Structured Illumination Microscopy (SIM) for research in Immunology. This allowed the publication of the first super-resolution images of endogenous B cell receptors, showing that these receptors are pre-clustered before B-cell activation. He improved the depth penetration for two-photon microscopy in lymph nodes utilising this technique for imaging of iNKT cells. His research at Cambridge University was focussed on implementing Single Molecule Tracking in Total Internal Fluorescence Microscopy (TIRFM) in combination with the deposition of biomolecules from a nanopipette using Scanning Ion-conductance Microscopy (SICM).
et al., 2017, Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells., Cell, Vol:172, ISSN:0092-8674, Pages:517-533.e20
et al., 2017, A switch from canonical to noncanonical autophagy shapes B cell responses, Science, Vol:355, ISSN:0036-8075, Pages:641-647
et al., 2015, Inflammation-induced disruption of SCS macrophages impairs B cell responses to secondary infection, Science, Vol:347, ISSN:0036-8075, Pages:667-672
et al., 2013, The actin and tetraspanin networks organize receptor nanoclusters to regulate B cell receptor-mediated signaling., Immunity, Vol:38, Pages:461-474
et al., 2012, Asymmetric Segregation of Polarized Antigen on B Cell Division Shapes Presentation Capacity, Science, Vol:335, ISSN:0036-8075, Pages:475-479