Imperial College London
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DrAnneBurke-Gaffney

Faculty of Medicine, National Heart & Lung Institute

Lecturer
 
 
 
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Contact

 

a.burke-gaffney

 
 
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Location

 

GSB 311Royal BromptonRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Burke-Gaffney:2014:10.1371/journal.pone.0111459,
author = {Burke-Gaffney, A and Svermova, T and Mumby, S and Finney, SJ and Evans, TW},
doi = {10.1371/journal.pone.0111459},
journal = {PLOS One},
title = {Raised Plasma Robo4 and Cardiac Surgery-Associated Acute Kidney Injury},
url = {http://dx.doi.org/10.1371/journal.pone.0111459},
volume = {9},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Objective: Endothelial dysfunction associated with systemic inflammation can contribute to organ injury/failure followingcardiac surgery requiring cardiopulmonary bypass (CPB). Roundabout protein 4 (Robo4), an endothelial-expressedtransmembrane receptor and regulator of cell activation, is an important inhibitor of endothelial hyper-permeability. Weinvestigated the hypothesis that plasma levels of Robo4 are indicative of organ injury, in particular acute kidney injury (AKI),after cardiac surgery.Methods: Patients (n = 32) undergoing elective cardiac surgery with CPB were enrolled, prospectively. Plasma Robo4concentrations were measured pre-, 2 and 24 h post-operatively, using a commercially available ELISA. Plasma andendothelial markers of inflammation [interleukin (IL) -6, -8, -10: von Willibrand factor (vWF) and angiopoeitin-2 (Ang-2)] andthe AKI marker, neutrophil gelatinase-associated lipocalin (NGAL), were also measured by ELISA.Results: Plasma Robo4 increased significantly (p,0.001) from pre-operative levels of 25156904 pg/ml to 447361915 pg/ml, 2 h after surgery; and returned to basal levels (26826979 pg/ml) by 24 h. Plasma cytokines, vWF and NGAL alsoincreased 2 h post-operatively and remained elevated at 24 h. Ang-2 increased 24 h post-operatively, only. There was apositive, significant correlation (r = 0.385, p = 0.0298) between Robo-4 and IL-10, but not other cytokines, 2 h postoperatively.Whilst raised Robo4 did not correlate with indices of lung dysfunction or other biomarkers of endothelialactivation; there was a positive, significant correlation between raised (2 h) plasma NGAL and Robo4 (r = 0.4322, p = 0.0135).When patients were classed as AKI or non-AKI either using NGAL cut-off of 150 ng/ml, or the AKI Network (AKIN) clinicalclassification; plasma Robo4 was significantly higher (p = 0.0073 and 0.003, respectively) in AKI vs. non-AKI patients (NGALcut-off: 535062191 ng/ml, n = 16 vs. 359561068 pg/ml, n = 16; AKIN: 6546 pg/ml, IQR 5025–8079, n
AU  - Burke-Gaffney,A
AU  - Svermova,T
AU  - Mumby,S
AU  - Finney,SJ
AU  - Evans,TW
DO  - 10.1371/journal.pone.0111459
PY  - 2014///
SN  - 1932-6203
TI  - Raised Plasma Robo4 and Cardiac Surgery-Associated Acute Kidney Injury
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0111459
UR  - http://hdl.handle.net/10044/1/26497
VL  - 9
ER  -
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