Imperial College London
Alternate

Professor Alan C Spivey

Faculty of Natural Sciences, Department of Chemistry

Associate Provost (L&T), Professor of Synthetic Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5841a.c.spivey Website CV

 
 
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Location

 

501CMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Piletsky:2021:10.1016/j.nantod.2021.101304,
author = {Piletsky, S and Piletska, E and Poblocka, M and Macip, S and Jones, D and Braga, M and Cao, T and Singh, R and Spivey, A and Aboagye, E and Piletsky, S},
doi = {10.1016/j.nantod.2021.101304},
journal = {Nano Today: an international rapid reviews journal},
pages = {1--8},
title = {Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers},
url = {http://dx.doi.org/10.1016/j.nantod.2021.101304},
volume = {41},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps of cell surfaces, with the aim of identifying cell surface markers and epitopes for diagnostic and therapeutic applications. The analysis of two cancer cell lines, HN5 and MDA-MB-468, is described herein as a proof of concept, along with the selective targeting of three identified epitopes of epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were identified using this technique, with 283 considered to be transmembrane or extracellular proteins. The major advantage of the molecular imprinting approach developed here is the ability to analyse cell surface proteins without tedious fractionation, affinity separation or labelling. We believe that this system of protein analysis may provide a basic molecular diagnostics toolbox for precise, personalised treatment of cancer and other diseases.
AU  - Piletsky,S
AU  - Piletska,E
AU  - Poblocka,M
AU  - Macip,S
AU  - Jones,D
AU  - Braga,M
AU  - Cao,T
AU  - Singh,R
AU  - Spivey,A
AU  - Aboagye,E
AU  - Piletsky,S
DO  - 10.1016/j.nantod.2021.101304
EP  - 8
PY  - 2021///
SN  - 1748-0132
SP  - 1
TI  - Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers
T2  - Nano Today: an international rapid reviews journal
UR  - http://dx.doi.org/10.1016/j.nantod.2021.101304
UR  - https://www.sciencedirect.com/science/article/pii/S1748013221002292?via%3Dihub
UR  - http://hdl.handle.net/10044/1/92324
VL  - 41
ER  -
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