Imperial College London
Dr Anil Chandrashekran

DrAnilChandrashekran

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Associate
 
 
 
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Contact

 

+44 (0)20 7594 2101a.chandrashekran98

 
 
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Location

 

IRDBHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Alder:2010:10.1242/dev.048363,
author = {Alder, O and Lavial, F and Helness, A and Brookes, E and Pinho, S and Chandrashekran, A and Arnaud, P and Pombo, A and O'Neill, L and Azuara, V},
doi = {10.1242/dev.048363},
journal = {Development},
pages = {2483--2492},
title = {Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment},
url = {http://dx.doi.org/10.1242/dev.048363},
volume = {137},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Pluripotent cells develop within the inner cell mass of blastocysts, a mosaic of cells surrounded by an extra-embryonic layer, the trophectoderm. We show that a set of somatic lineage regulators (including Hox, Gata and Sox factors) that carry bivalent chromatin enriched in H3K27me3 and H3K4me2 are selectively targeted by Suv39h1-mediated H3K9me3 and de novo DNA methylation in extra-embryonic versus embryonic (pluripotent) lineages, as assessed both in blastocyst-derived stem cells and in vivo. This stably repressed state is linked with a loss of gene priming for transcription through the exclusion of PRC1 (Ring1B) and RNA polymerase II complexes at bivalent, lineage-inappropriate genes upon trophoblast lineage commitment. Collectively, our results suggest a mutually exclusive role for Ring1B and Suv39h1 in regulating distinct chromatin states at key developmental genes and propose a novel mechanism by which lineage specification can be reinforced during early development.
AU  - Alder,O
AU  - Lavial,F
AU  - Helness,A
AU  - Brookes,E
AU  - Pinho,S
AU  - Chandrashekran,A
AU  - Arnaud,P
AU  - Pombo,A
AU  - O'Neill,L
AU  - Azuara,V
DO  - 10.1242/dev.048363
EP  - 2492
PY  - 2010///
SN  - 0950-1991
SP  - 2483
TI  - Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment
T2  - Development
UR  - http://dx.doi.org/10.1242/dev.048363
UR  - http://hdl.handle.net/10044/1/23490
VL  - 137
ER  -
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