Imperial College London
Alternate

Dr Abigail Clements

Faculty of Natural Sciences, Department of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7681a.clements

 
 
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Location

 

1.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jégouzo:2020:10.1074/jbc.RA120.012783,
author = {Jégouzo, SAF and Nelson, C and Hardwick, T and Wong, STA and Lau, NKK and Neoh, GKE and Castellanos-Rueda, R and Huang, Z and Mignot, B and Hirdaramani, A and Howitt, A and Frewin, K and Shen, Z and Fox, RJ and Wong, R and Ando, M and Emony, L and Zhu, H and Holder, A and Werling, D and Krishnan, N and Robertson, BD and Clements, A and Taylor, ME and Drickamer, K},
doi = {10.1074/jbc.RA120.012783},
journal = {Journal of Biological Chemistry},
pages = {4541--4555},
title = {Mammalian lectin arrays for screening host–microbe interactions},
url = {http://dx.doi.org/10.1074/jbc.RA120.012783},
volume = {295},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Many members of the C-type lectin family of glycan-binding receptors have been ascribed roles in the recognition of microorganisms and serve as key receptors in the innate immune response to pathogens. Other mammalian receptors have become targets through which pathogens enter target cells. These receptor roles have often been documented with binding studies involving individual pairs of receptors and microorganisms. To provide a systematic overview of interactions between microbes and the large complement of C-type lectins, here we developed a lectin array and suitable protocols for labeling of microbes that could be used to probe this array. The array contains C-type lectins from cow, chosen as a model organism of agricultural interest for which the relevant pathogen–receptor interactions have not been previously investigated in detail. Screening with yeast cells and various strains of both Gram-positive and -negative bacteria revealed distinct binding patterns, which in some cases could be explained by binding to lipopolysaccharides or capsular polysaccharides, but in other cases they suggested the presence of novel glycan targets on many of the microorganisms. These results are consistent with interactions previously ascribed to the receptors, but they also highlight binding to additional sugar targets that have not previously been recognized. Our findings indicate that mammalian lectin arrays represent unique discovery tools for identifying both novel ligands and new receptor functions.
AU  - Jégouzo,SAF
AU  - Nelson,C
AU  - Hardwick,T
AU  - Wong,STA
AU  - Lau,NKK
AU  - Neoh,GKE
AU  - Castellanos-Rueda,R
AU  - Huang,Z
AU  - Mignot,B
AU  - Hirdaramani,A
AU  - Howitt,A
AU  - Frewin,K
AU  - Shen,Z
AU  - Fox,RJ
AU  - Wong,R
AU  - Ando,M
AU  - Emony,L
AU  - Zhu,H
AU  - Holder,A
AU  - Werling,D
AU  - Krishnan,N
AU  - Robertson,BD
AU  - Clements,A
AU  - Taylor,ME
AU  - Drickamer,K
DO  - 10.1074/jbc.RA120.012783
EP  - 4555
PY  - 2020///
SN  - 0021-9258
SP  - 4541
TI  - Mammalian lectin arrays for screening host–microbe interactions
T2  - Journal of Biological Chemistry
UR  - http://dx.doi.org/10.1074/jbc.RA120.012783
UR  - https://www.sciencedirect.com/science/article/pii/S0021925817487187?via%3Dihub
UR  - http://hdl.handle.net/10044/1/78165
VL  - 295
ER  -
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