Imperial College London
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Dr Ana P. Costa-Pereira

Central Faculty, Centre for Languages, Culture and Communication

Director of Centre for Languages, Culture & Communication
 
 
 
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Contact

 

+44 (0)20 7594 9352a.costa-pereira Website

 
 
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Assistant

 

Mrs Sheila Ekudo +44 (0)20 7594 2086

 
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Location

 

S302Sherfield BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Costa-Pereira:2013,
author = {Costa-Pereira, AP},
booktitle = {Transmembrane Signaling Protocols},
editor = {Dhanasekaran},
publisher = {The Humana Press Inc.},
title = {Cytokine Responses Profiling Using Flow Cytometry-Based Kinome Analyses},
url = {http://www1.imperial.ac.uk/medicine/people/a.costa-pereira/},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - RNA interference (RNAi) has revolutionised the way we interrogate the role of particular molecules in specific molecular processes. Unsurprisingly, its discovery awarded the Nobel Prize of Medicine and Physiology in 2006 to Craig Mello and Andrew Fire, a mere eight years after they stumbled upon it (1). RNAi can be induced using short interfering (si) or short hairpin (sh) RNA molecules and it permits the downregulation of a specific protein by interfering/ degrading its corresponding mRNA. siRNA molecules are typically 21-23 nucleotide long, an important characteristic, as longer dsRNAs are interpreted by mammalian cells as intermediaries of a viral infection, which quickly activate an antiviral response leading to their destruction (2). Indeed, up to 2001 this phenomenon was ‘easily’ achievable in a variety of organisms such as D. melanogaster and C. elegans but remained a challenge in mammalian cells until Tuschl and co-workers (2) managed to successfully induce RNAi in cultured mammalian cells without significantly triggering the Interferon (IFN) system.Despite high redundancy in mammalian cells, RNAi screens using siRNA molecules offer tremendous potential to identify new players in well-defined pathways (3). This chapter will describe in great depth flow cytometry-based kinome-wide siRNA screens designed to identify additional molecules involved in signalling triggered downstream of the IFN-γ and IFN-α receptors (IFNGR and IFNAR, respectively).
AU  - Costa-Pereira,AP
PB  - The Humana Press Inc.
PY  - 2013///
TI  - Cytokine Responses Profiling Using Flow Cytometry-Based Kinome Analyses
T1  - Transmembrane Signaling Protocols
UR  - http://www1.imperial.ac.uk/medicine/people/a.costa-pereira/
ER  -
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