Imperial College London
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Dr Ana P. Costa-Pereira

Central Faculty, Centre for Languages, Culture and Communication

Director of Centre for Languages, Culture & Communication
 
 
 
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Contact

 

+44 (0)20 7594 9352a.costa-pereira Website

 
 
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Assistant

 

Mrs Sheila Ekudo +44 (0)20 7594 2086

 
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Location

 

S302Sherfield BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rutherford:2016:10.1126/scisignal.aaf8566,
author = {Rutherford, C and Speirs, C and Williams, JJL and Ewart, MA and Mancini, SJ and Hawley, SA and Delles, C and Viollet, B and Costa-Pereira, AP and Baillie, GS and Salt, IP and Palmer, TM},
doi = {10.1126/scisignal.aaf8566},
journal = {Science Signaling},
title = {Phosphorylation of Janus kinase 1 (JAK1) by AMP-activated protein kinase (AMPK) links energy sensing to anti-inflammatory signaling},
url = {http://dx.doi.org/10.1126/scisignal.aaf8566},
volume = {9},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Adenosine 5′-monophosphate–activated protein kinase (AMPK) is a pivotal regulator of metabolism at cellular and organismal levels. AMPK also suppresses inflammation. We found that pharmacological activation of AMPK rapidly inhibited the Janus kinase (JAK)–signal transducer and activator of transcription (STAT) pathway in various cells. In vitro kinase assays revealed that AMPK directly phosphorylated two residues (Ser515 and Ser518) within the Src homology 2 domain of JAK1. Activation of AMPK enhanced the interaction between JAK1 and 14-3-3 proteins in cultured vascular endothelial cells and fibroblasts, an effect that required the presence of Ser515 and Ser518 and was abolished in cells lacking AMPK catalytic subunits. Mutation of Ser515 and Ser518 abolished AMPK-mediated inhibition of JAK-STAT signaling stimulated by either the sIL-6Rα/IL-6 complex or the expression of a constitutively active V658F-mutant JAK1 in human fibrosarcoma cells. Clinically used AMPK activators metformin and salicylate enhanced the inhibitory phosphorylation of endogenous JAK1 and inhibited STAT3 phosphorylation in primary vascular endothelial cells. Therefore, our findings reveal a mechanism by which JAK1 function and inflammatory signaling may be suppressed in response to metabolic stress and provide a mechanistic rationale for the investigation of AMPK activators in a range of diseases associated with enhanced activation of the JAK-STAT pathway.
AU  - Rutherford,C
AU  - Speirs,C
AU  - Williams,JJL
AU  - Ewart,MA
AU  - Mancini,SJ
AU  - Hawley,SA
AU  - Delles,C
AU  - Viollet,B
AU  - Costa-Pereira,AP
AU  - Baillie,GS
AU  - Salt,IP
AU  - Palmer,TM
DO  - 10.1126/scisignal.aaf8566
PY  - 2016///
SN  - 1945-0877
TI  - Phosphorylation of Janus kinase 1 (JAK1) by AMP-activated protein kinase (AMPK) links energy sensing to anti-inflammatory signaling
T2  - Science Signaling
UR  - http://dx.doi.org/10.1126/scisignal.aaf8566
UR  - http://hdl.handle.net/10044/1/43027
VL  - 9
ER  -
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