Imperial College London
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Dr Ana P. Costa-Pereira

Central Faculty, Centre for Languages, Culture and Communication

Director of Centre for Languages, Culture & Communication
 
 
 
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Contact

 

+44 (0)20 7594 9352a.costa-pereira Website

 
 
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Assistant

 

Mrs Sheila Ekudo +44 (0)20 7594 2086

 
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Location

 

S302Sherfield BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hergovits:2017:10.1111/jcmm.13221,
author = {Hergovits, S and Mais, C and Haan, C and Costa-Pereira, AP and Hermanns, HM},
doi = {10.1111/jcmm.13221},
journal = {Journal of Cellular and Molecular Medicine},
pages = {3087--3099},
title = {Oncostatin M induces RIG-I and MDA5 expression and enhances the double-stranded RNA response in fibroblasts},
url = {http://dx.doi.org/10.1111/jcmm.13221},
volume = {21},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Interleukin (IL)-6-type cytokines have no direct antiviral activity; nevertheless, they display immune-modulatory functions. Oncostatin M (OSM), a member of the IL-6 family, has recently been shown to induce a distinct number of classical interferon stimulated genes (ISG). Most of them are involved in antigen processing and presentation. However, induction of retinoic acid-inducible gene (RIG)-I-like receptors (RLR) has not been investigated. Here we report that OSM has the capability to induce the expression of the DExD/H-Box RNA helicases RIG-I and melanoma differentiation antigen 5 (MDA5) as well as of the transcription factors interferon regulatory factor (IRF)1, IRF7 and IRF9 in primary fibroblasts. Induction of the helicases depends on tyrosine as well as serine phosphorylation of STAT1. Moreover, we could show that the OSM-induced STAT1 phosphorylation is predominantly counter-regulated by a strong STAT3-dependent SOCS3 induction, as Stat3 as well as Socs3 knock-down results in an enhanced and prolonged helicase and IRF expression. Other factors involved in regulation of STAT1 or IRF1 activity, like protein tyrosine phosphatase, non-receptor type 2 (PTPN2), promyelocytic leukaemia protein (PML) or small ubiquitin-related modifier 1 (SUMO1), play a minor role in OSM-mediated induction of RLR. Remarkably, OSM and interferon-γ (IFN-γ) synergize to mediate transcription of RLR and pre-treatment of fibroblasts with OSM fosters the type I interferon production in response to a subsequent encounter with double-stranded RNA. Together, these findings suggest that the OSM-induced JAK/STAT1 signalling is implicated in virus protection of non-professional immune cells and may cooperate with interferons to enhance RLR expression in these cells.
AU  - Hergovits,S
AU  - Mais,C
AU  - Haan,C
AU  - Costa-Pereira,AP
AU  - Hermanns,HM
DO  - 10.1111/jcmm.13221
EP  - 3099
PY  - 2017///
SN  - 1582-4934
SP  - 3087
TI  - Oncostatin M induces RIG-I and MDA5 expression and enhances the double-stranded RNA response in fibroblasts
T2  - Journal of Cellular and Molecular Medicine
UR  - http://dx.doi.org/10.1111/jcmm.13221
UR  - http://hdl.handle.net/10044/1/49313
VL  - 21
ER  -
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