Imperial College London
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ProfessorAdnanCustovic

Faculty of Medicine, National Heart & Lung Institute

Professor of Paediatric Allergy
 
 
 
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a.custovic CV

 
 
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Location

 

220Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Broadbent:2020:10.1371/journal.pone.0228229,
author = {Broadbent, L and Manzoor, S and Zarcone, MC and Barabas, J and Shields, MD and Saglani, S and Lloyd, CM and Bush, A and Custovic, A and Ghazal, P and Gore, M and Marsland, B and Roberts, G and Schwarze, J and Turner, S and Power, UF},
doi = {10.1371/journal.pone.0228229},
journal = {PLoS One},
pages = {1--12},
title = {Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media},
url = {http://dx.doi.org/10.1371/journal.pone.0228229},
volume = {15},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The culture of differentiated human airway epithelial cells allows the study of pathogen-host interactions and innate immune responses in a physiologically relevant in vitro model. As the use of primary cell culture has gained popularity the availability of the reagents needed to generate these cultures has increased. In this study we assessed two different media, Promocell and PneumaCult, during the differentiation and maintenance of well-differentiated primary nasal epithelial cell cultures (WD-PNECs). We compared and contrasted the consequences of these media on WD-PNEC morphological and physiological characteristics and their responses to respiratory syncytial virus (RSV) infection. We found that cultures generated using PneumaCult resulted in greater total numbers of smaller, tightly packed, pseudostratified cells. However, cultures from both media resulted in similar proportions of ciliated and goblet cells. There were no differences in RSV growth kinetics, although more ciliated cells were infected in the PneumaCult cultures. There was also significantly more IL-29/IFNλ1 secreted from PneumaCult compared to Promocell cultures following infection. In conclusion, the type of medium used for the differentiation of primary human airway epithelial cells may impact experimental results.
AU  - Broadbent,L
AU  - Manzoor,S
AU  - Zarcone,MC
AU  - Barabas,J
AU  - Shields,MD
AU  - Saglani,S
AU  - Lloyd,CM
AU  - Bush,A
AU  - Custovic,A
AU  - Ghazal,P
AU  - Gore,M
AU  - Marsland,B
AU  - Roberts,G
AU  - Schwarze,J
AU  - Turner,S
AU  - Power,UF
DO  - 10.1371/journal.pone.0228229
EP  - 12
PY  - 2020///
SN  - 1932-6203
SP  - 1
TI  - Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0228229
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000535934200004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228229
UR  - http://hdl.handle.net/10044/1/81157
VL  - 15
ER  -
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