Imperial College London
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ProfessorAlfonsoDe Simone

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3052a.de-simon Website

 
 
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Location

 

603Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Qu:2019:10.1042/BCJ20190779,
author = {Qu, F and ElOmari, K and Wagner, A and De, Simone A and Beis, K},
doi = {10.1042/BCJ20190779},
journal = {Biochemical Journal},
pages = {3649--3660},
title = {Desolvation of the substrate binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC},
url = {http://dx.doi.org/10.1042/BCJ20190779},
volume = {476},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Under limiting sulfur availability, bacteria can assimilate sulfur from alkanesulfonates. Bacteria utilize ATP-binding cassette (ABC) transporters to internalise them for further processing to release sulfur. In gram-negative bacteria the TauABC and SsuABC ensure internalization, although, these two systems have common substrates, the former has been characterised as a taurine specific system. TauA and SsuA are substrate binding proteins (SBPs) that bind and bring the alkanesulfonates to the ABC importer for transport. Here, we have determined the crystal structure of TauA and have characterised its thermodynamic binding parameters by isothermal titration calorimetry in complex with taurine and different alkanesulfonates. Our structures revealed that the coordination of the alkanesulfonates is conserved, with the exception of Asp205 that is absent in SsuA, but the thermodynamic parameters revealed a very high enthalpic penalty cost for binding of the other alkanesulfonates relative to taurine. Our molecular dynamic simulations indicated that the different levels of hydration of the binding site contributed to the selectivity for taurine over the other alkanesulfonates. Such selectivity mechanism is very likely to be employed by other SBPs of ABC transporters.
AU  - Qu,F
AU  - ElOmari,K
AU  - Wagner,A
AU  - De,Simone A
AU  - Beis,K
DO  - 10.1042/BCJ20190779
EP  - 3660
PY  - 2019///
SN  - 0264-6021
SP  - 3649
TI  - Desolvation of the substrate binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC
T2  - Biochemical Journal
UR  - http://dx.doi.org/10.1042/BCJ20190779
UR  - https://portlandpress.com/biochemj/article/doi/10.1042/BCJ20190779/221299/Desolvation-of-the-substrate-binding-protein-TauA
UR  - http://hdl.handle.net/10044/1/75388
VL  - 476
ER  -
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