Publications
330 results found
Babu P, North SJ, Jang-Lee J, et al., 2009, Structural characterisation of neutrophil glycans by ultra sensitive mass spectrometric glycomics methodology, GLYCOCONJUGATE JOURNAL, Vol: 26, Pages: 975-986, ISSN: 0282-0080
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- Citations: 60
North SJ, Hitchen PG, Haslam SM, et al., 2009, Mass spectrometry in the analysis of <i>N</i>-linked and <i>O</i>-linked glycans, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 19, Pages: 498-506, ISSN: 0959-440X
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- Citations: 166
Montpetit ML, Stocker PJ, Schwetz TA, et al., 2009, Regulated and aberrant glycosylation modulate cardiac electrical signaling, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 16517-16522, ISSN: 0027-8424
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- Citations: 84
Chan RWY, Chan MCW, Wong ACN, et al., 2009, DAS181 Inhibits H5N1 Influenza Virus Infection of Human Lung Tissues, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol: 53, Pages: 3935-3941, ISSN: 0066-4804
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- Citations: 53
Powlesland AS, Hitchen PG, Parry S, et al., 2009, Targeted glycoproteomic identification of cancer cell glycosylation, GLYCOBIOLOGY, Vol: 19, Pages: 899-909, ISSN: 0959-6658
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- Citations: 58
Pang P, Tissot B, Drobnis EZ, et al., 2009, Analysis of the Human Seminal Plasma Glycome Reveals the Presence of Immunomodulatory Carbohydrate Functional Groups, Journal of Proteome Research, Vol: 8, Pages: 4906-4915, ISSN: 1535-3893
Stone EL, Ismail MN, Lee SH, et al., 2009, Glycosyltransferase Function in Core 2-Type Protein O Glycosylation, MOLECULAR AND CELLULAR BIOLOGY, Vol: 29, Pages: 3770-3782, ISSN: 0270-7306
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- Citations: 84
Mitoma J, Miyazaki T, Sutton-Smith M, et al., 2009, The <i>N</i>-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells, GLYCOCONJUGATE JOURNAL, Vol: 26, Pages: 511-523, ISSN: 0282-0080
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- Citations: 24
Tissot B, North SJ, Ceroni A, et al., 2009, Glycoproteomics: Past, present and future, FEBS Letters, Vol: 583, Pages: 1728-1735, ISSN: 0014-5793
This invited paper reviews the study of protein glycosylation, commonly known as glycoproteomics, beginning with the origins of the subject area in the early 1970s shortly after mass spectrometry was first applied to protein sequencing. We go on to describe current analytical approaches to glycoproteomic analyses, with exemplar projects presented in the form of the complex story of human glycodelin and the characterisation of blood group H eptitopes on the O-glycans of gp273 from Unio elongatulus. Finally, we present an update on the latest progress in the field of automated and semi-automated interpretation and annotation of these data in the form of GlycoWorkBench, a powerful informatics tool that provides valuable assistance in unravelling the complexities of glycoproteomic studies.
Lee C, Pang P, Yeung WSB, et al., 2009, Effects of Differential Glycosylation of Glycodelins on Lymphocyte Survival, Journal of Biological Chemistry, Vol: 284, Pages: 15084-15096
Glycodelin is a human glycoprotein with four reported glycoforms, namely glycodelin-A (GdA), glycodelin-F (GdF), glycodelin-C (GdC), and glycodelin-S (GdS). These glycoforms have the same protein core and appear to differ in their N-glycosylation. The glycosylation of GdA is completely different from that of GdS. GdA inhibits proliferation and induces cell death of T cells. However, the glycosylation and immunomodulating activities of GdF and GdC are not known. This study aimed to use ultra-high sensitivity mass spectrometry to compare the glycomes of GdA, GdC, and GdF and to study the relationship between the immunological activity and glycosylation pattern among glycodelin glycoforms. Using MALDI-TOF strategies, the glycoforms were shown to contain an enormous diversity of bi-, tri-, and tetra-antennary complex-type glycans carrying Galβ1–4GlcNAc (lacNAc) and/or GalNAcβ1–4GlcNAc (lacdiNAc) antennae backbones with varying levels of fucose and sialic acid substitution. Interestingly, they all carried a family of Sda (NeuAcα2–3(GalNAcβ1–4)Gal)-containing glycans, which were not identified in the earlier study because of less sensitive methodologies used. Among the three glycodelins, GdA is the most heavily sialylated. Virtually all the sialic acid on GdC is located on the Sda antennae. With the exception of the Sda epitope, the GdC N-glycome appears to be the asialylated counterpart of the GdA/GdF glycomes. Sialidase activity, which may be responsible for transforming GdA/GdF to GdC, was detected in cumulus cells. Both GdA and GdF inhibited the proliferation, induced cell death, and suppressed interleukin-2 secretion of Jurkat cells and peripheral blood mononuclear cells. In contrast, no immunosuppressive effect was observed for GdS and GdC.
Tabei SMB, Hitchen PG, Day-Williams MJ, et al., 2009, An Aeromonas caviae Genomic Island Is Required for both O-Antigen Lipopolysaccharide Biosynthesis and Flagellin Glycosylation, Journal of Bacteriology, Vol: 191, Pages: 2851-2863
Aeromonas caviae Sch3N possesses a small genomic island that is involved in both flagellin glycosylation and lipopolysaccharide (LPS) O-antigen biosynthesis. This island appears to have been laterally acquired as it is flanked by insertion element-like sequences and has a much lower G+C content than the average aeromonad G+C content. Most of the gene products encoded by the island are orthologues of proteins that have been shown to be involved in pseudaminic acid biosynthesis and flagellin glycosylation in both Campylobacter jejuni and Helicobacter pylori. Two of the genes, lst and lsg, are LPS specific as mutation of them results in the loss of only a band for the LPS O-antigen. Lsg encodes a putative Wzx flippase, and mutation of Lsg affects only LPS; this finding supports the notion that flagellin glycosylation occurs within the cell before the flagellins are exported and assembled and not at the surface once the sugar has been exported. The proteins encoded by flmA, flmB, neuA, flmD, and neuB are thought to make up a pseudaminic acid biosynthetic pathway, and mutation of any of these genes resulted in the loss of motility, flagellar expression, and a band for the LPS O-antigen. Furthermore, pseudaminic acid was shown to be present on both flagellin subunits that make up the polar flagellum filament, to be present in the LPS O-antigen of the A. caviae wild-type strain, and to be absent from the A. caviae flmD mutant strain.
Pang PC, Tissot B, Drobnis EZ, et al., 2009, GLYCOMIC ANALYSIS OF HUMAN SEMINAL PLASMA REVEALS THE PRESENCE OF IMMUNOMODULATORY CARBOHYDRATE FUNCTIONAL GROUPS, 34th Annual Meeting of the American-Society-of-Andrology, Publisher: AMER SOC ANDROLOGY, INC, Pages: 54-55, ISSN: 0196-3635
Qazi O, Hitchen P, Tissot B, et al., 2009, Mass spectrometric analysis of the S-layer proteins from <i>Clostridium difficile</i> demonstrates the absence of glycosylation, JOURNAL OF MASS SPECTROMETRY, Vol: 44, Pages: 368-374, ISSN: 1076-5174
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- Citations: 16
Goldberg D, Bern M, North SJ, et al., 2009, Glycan family analysis for deducing <i>N</i>-glycan topology from single MS, BIOINFORMATICS, Vol: 25, Pages: 365-371, ISSN: 1367-4803
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- Citations: 23
Wehmeier S, Varghese AS, Gurcha SS, et al., 2009, Glycosylation of the phosphate binding protein, PstS, in <i>Streptomyces coelicolor</i> by a pathway that resembles protein O-mannosylation in eukaryotes, MOLECULAR MICROBIOLOGY, Vol: 71, Pages: 421-433, ISSN: 0950-382X
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- Citations: 44
North SJ, Chalabi S, Sutton-Smith M, et al., 2009, Mouse and Human Glycomics, Handbook of glycomics, Editors: Cummings, Pierce, Publisher: Academic Press, ISBN: 9780123736000
Key Features
Tissot B, Ceroni A, Powell AK, et al., 2008, Software Tool for the Structural Determination of Glycosaminoglycans by Mass Spectrometry, ANALYTICAL CHEMISTRY, Vol: 80, Pages: 9204-9212, ISSN: 0003-2700
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- Citations: 31
Huang L, Hollingsworth RI, Haslam SM, et al., 2008, The Lan3-2 glycoepitope of <i>Hirudo medicinalis</i> consists of β-(1,4)-linked mannopyranose Cell type-specific glycans of <i>H-medicinalis</i>, JOURNAL OF NEUROCHEMISTRY, Vol: 107, Pages: 1448-1456, ISSN: 0022-3042
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- Citations: 3
Ismail MN, Stone EL, Lee SH, et al., 2008, High Sensitivity O-Glycomic Analysis of Core 2 GlcNAc Transferase Knockout Mice, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 969-969, ISSN: 0959-6658
Clark GF, Azadi P, Ishihara M, et al., 2008, Propylaminated Biotinylglycans: Useful Reagents for Glycobiological Investigations, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 983-983, ISSN: 0959-6658
Dell A, Haslam S, 2008, Towards Sytems Glycobiology: Glycomic and Glycoproteomic Strategies, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 940-940, ISSN: 0959-6658
Stone EL, Ismail MN, Lee SH, et al., 2008, Investigating the Structure and Function of O-Glycans in Core 2 GnT Deficiency, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 960-960, ISSN: 0959-6658
Haslam SM, Julien S, Burchell JM, et al., 2008, Characterizing the glycome of the mammalian immune system, IMMUNOLOGY AND CELL BIOLOGY, Vol: 86, Pages: 564-573, ISSN: 0818-9641
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- Citations: 49
Steiner K, Hanreich A, Kainz B, et al., 2008, Recombinant Glycans on an S-Layer Self-Assembly Protein: A New Dimension for Nanopatterned Biomaterials, SMALL, Vol: 4, Pages: 1728-1740, ISSN: 1613-6810
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- Citations: 21
Yurist-Doutsch S, Abu-Qarn M, Battaglia F, et al., 2008, <i>aglF</i>, <i>aglG</i> and <i>aglI</i>, novel members of a gene island involved in the N-glycosylation of the <i>Haloferax volcanii</i> S-layer glycoprotein, MOLECULAR MICROBIOLOGY, Vol: 69, Pages: 1234-1245, ISSN: 0950-382X
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- Citations: 60
Mishra AK, Klein C, Gurcha SS, et al., 2008, Structural characterization and functional properties of a novel lipomannan variant isolated from a <i>Corynebacterium glutamicum pimB</i>′ mutant, ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, Vol: 94, Pages: 277-287, ISSN: 0003-6072
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- Citations: 27
Aleksic M, Pease CK, Basketter DA, et al., 2008, Mass spectrometric identification of covalent adducts of the skin allergen 2,4-dinitro-1-chlorobenzene and model skin proteins, TOXICOLOGY IN VITRO, Vol: 22, Pages: 1169-1176, ISSN: 0887-2333
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- Citations: 35
Sun W, Parry S, Panico M, et al., 2008, <i>N</i>-glycans and the N terminus of protein C inhibitor affect the cofactor-enhanced rates of thrombin inhibition, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 283, Pages: 18601-18611
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- Citations: 17
Wei C-J, Xu L, Kong W-P, et al., 2008, Comparative efficacy of neutralizing antibodies elicited by recombinant hemagglutinin proteins from avian H5N1 influenza virus, JOURNAL OF VIROLOGY, Vol: 82, Pages: 6200-6208, ISSN: 0022-538X
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- Citations: 128
Shi W, Huang Y, Sutton-Smith M, et al., 2008, A filovirus-unique region of Ebola virus nucleoprotein confers aberrant migration and mediates its incorporation into virions, JOURNAL OF VIROLOGY, Vol: 82, Pages: 6190-6199, ISSN: 0022-538X
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- Citations: 31
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