Imperial College London
Dr Aarif O Eifan

Dr Aarif O Eifan

Faculty of Medicine, National Heart & Lung Institute

Clinical Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3164a.eifan Website

 
 
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Location

 

Royal BromptonRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Scadding:2015:10.1111/cea.12434,
author = {Scadding, GW and Eifan, A and Penagos, M and Dumitru, A and Switzer, A and McMahon, O and Phippard, D and Togias, A and Durham, SR and Shamji, MH},
doi = {10.1111/cea.12434},
journal = {Clinical and Experimental Allergy},
pages = {613--623},
title = {Local and systemic effects of cat allergen nasal provocation},
url = {http://dx.doi.org/10.1111/cea.12434},
volume = {45},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundCat allergen is widely distributed in homes and schools; allergic sensitization is common.ObjectiveTo develop a model of cat allergen nasal challenge to establish dose–response and time–course characteristics and investigate local and systemic biomarkers of allergic inflammation.MethodsNineteen catallergic individuals underwent titrated nasal challenge, range 0.243 to 14.6 μg/mL Fel d1, and matched diluentonly provocation. Clinical response to 8 h was assessed by symptom scores and peak nasal inspiratory flow (PNIF). Nasal fluid was collected using polyurethane sponges and analysed by ImmunoCAP and multiplex assays. Whole blood flow cytometry for basophil surface CD63, CD107a, and CD203c was carried out at baseline and 6 h postchallenge.ResultsA dose–response to allergen was seen in symptom scores and PNIF, maximal at 10 000 BU/mL (4.87 μg/mL Fel d1), P < 0.0001 vs. diluent. Nasal fluid tryptase was elevated at 5 min after challenge (P < 0.05 vs. diluent); eotaxin, IL4, 5, 9, and 13 were increased at 8 h (P < 0.05 to P < 0.0001 vs. diluent); TSLP was undetectable; IL10, IL17A, and IL33 were unchanged compared to diluent challenge. Nasal fluid IL5 and IL13 correlated inversely with PNIF after challenge (IL5, r = −0.79, P < 0.0001; IL13, r = −0.60, P = 0.006). Surface expression of CD63 and CD107a was greater at 6 h than at baseline, both in the presence (both P < 0.05) and absence (CD63, P < 0.01; CD107a, P < 0.05) of in vitro allergen stimulation; no changes were seen on diluent challenge day.ConclusionsCat allergen nasal challenge produces local and systemic Th2driven inflammatory responses and has potential as a surrogate outcome measure in clinical trials.
AU  - Scadding,GW
AU  - Eifan,A
AU  - Penagos,M
AU  - Dumitru,A
AU  - Switzer,A
AU  - McMahon,O
AU  - Phippard,D
AU  - Togias,A
AU  - Durham,SR
AU  - Shamji,MH
DO  - 10.1111/cea.12434
EP  - 623
PY  - 2015///
SN  - 0954-7894
SP  - 613
TI  - Local and systemic effects of cat allergen nasal provocation
T2  - Clinical and Experimental Allergy
UR  - http://dx.doi.org/10.1111/cea.12434
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000350455800007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/cea.12434
UR  - http://hdl.handle.net/10044/1/82731
VL  - 45
ER  -
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