Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Bernal:2020:10.1101/2020.04.29.069310,
author = {Bernal, P and Furniss, C and Fecht, S and Leung, RCY and Spiga, L and Mavridou, DAI and Filloux, A},
doi = {10.1101/2020.04.29.069310},
publisher = {bioRxiv},
title = {Novel structural components generate distinct type VI secretion system anchoring modes},
url = {http://dx.doi.org/10.1101/2020.04.29.069310},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - The type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighboring cells. Despite their key function, TssA proteins exhibit unexpected diversity and exist in two major forms, a short (TssA S ) and a long (TssA L ) TssA. Whilst TssA L proteins interact with a partner, called TagA, to anchor the distal end of the extended sheath, the mechanism for the stabilization of TssA S -containing T6SSs remains unknown. Here we discover a novel class of structural components that interact with short TssA proteins and contribute to T6SS assembly by stabilizing the polymerizing sheath from the baseplate. We demonstrate that the presence of these components is important for full sheath extension and optimal firing. Moreover, we show that the pairing of each form of TssA with a different class of sheath stabilization proteins results in T6SS apparatuses that either reside in the cell for a while or fire immediately after sheath extension, thus giving rise to different aggression behaviors. We propose that this functional diversity could contribute to the specialization of the T6SS to suit bacterial lifestyles in diverse environmental niches.
AU  - Bernal,P
AU  - Furniss,C
AU  - Fecht,S
AU  - Leung,RCY
AU  - Spiga,L
AU  - Mavridou,DAI
AU  - Filloux,A
DO  - 10.1101/2020.04.29.069310
PB  - bioRxiv
PY  - 2020///
TI  - Novel structural components generate distinct type VI secretion system anchoring modes
UR  - http://dx.doi.org/10.1101/2020.04.29.069310
UR  - http://hdl.handle.net/10044/1/88184
ER  -
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