Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Steinchen:2021:10.1111/mmi.14684,
author = {Steinchen, W and Ahmad, S and Valentini, M and Eilers, K and Majkini, M and Altegoer, F and Lechner, M and Filloux, A and Whitney, JC and Bange, G},
doi = {10.1111/mmi.14684},
journal = {Molecular Microbiology},
pages = {1339--1356},
title = {Dual role of a (p)ppGpp- and (p)ppApp-degrading enzyme in biofilm formation and interbacterial antagonism},
url = {http://dx.doi.org/10.1111/mmi.14684},
volume = {115},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The guanosine nucleotidebased second messengers ppGpp and pppGpp (collectively: (p)ppGpp) enable adaptation of microorganisms to environmental changes and stress conditions. In contrast, the closely related adenosine nucleotides (p)ppApp are involved in type VI secretion system (T6SS)mediated killing during bacterial competition. Long RelASpoT Homolog (RSH) enzymes regulate synthesis and degradation of (p)ppGpp (and potentially also (p)ppApp) through their synthetase and hydrolase domains, respectively. Small alarmone hydrolases (SAH) that consist of only a hydrolase domain are found in a variety of bacterial species, including the opportunistic human pathogen Pseudomonas aeruginosa. Here, we present the structure and mechanism of P. aeruginosa SAH showing that the enzyme promiscuously hydrolyses (p)ppGpp and (p)ppApp in a strictly manganesedependent manner. While being dispensable for P. aeruginosa growth or swimming, swarming, and twitching motilities, its enzymatic activity is required for biofilm formation. Moreover, (p)ppAppdegradation by SAH provides protection against the T6SS (p)ppApp synthetase effector Tas1, suggesting that SAH enzymes can also serve as defense proteins during interbacterial competition.
AU  - Steinchen,W
AU  - Ahmad,S
AU  - Valentini,M
AU  - Eilers,K
AU  - Majkini,M
AU  - Altegoer,F
AU  - Lechner,M
AU  - Filloux,A
AU  - Whitney,JC
AU  - Bange,G
DO  - 10.1111/mmi.14684
EP  - 1356
PY  - 2021///
SN  - 0950-382X
SP  - 1339
TI  - Dual role of a (p)ppGpp- and (p)ppApp-degrading enzyme in biofilm formation and interbacterial antagonism
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.14684
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000611237200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1111/mmi.14684
UR  - http://hdl.handle.net/10044/1/88454
VL  - 115
ER  -
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