Imperial College London
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ProfessorAlainFilloux

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 9651a.filloux Website CV

 
 
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Location

 

1.47Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nolan:2021:10.1038/s41564-021-00950-8,
author = {Nolan, LM and Cain, AK and Clamens, T and Furniss, RCD and Manoli, E and Sainz-Polo, MA and Dougan, G and Albesa-Jove, D and Parkhill, J and Mavridou, DAI and Filloux, A},
doi = {10.1038/s41564-021-00950-8},
journal = {Nature Microbiology},
pages = {1199--+},
title = {Identification of tse8 as a type VI secretion system toxin from pseudomonas aeruginosa that targets the bacterial transamidosome to inhibit protein synthesis in prey cells},
url = {http://dx.doi.org/10.1038/s41564-021-00950-8},
volume = {6},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Type VI secretion system (T6SS) is a bacterial nanomachine that delivers toxic effectors to kill competitors or subvert some of their key functions. Here, we use transposon directed insertion–site sequencing to identify T6SS toxins associated with the H1-T6SS, one of the three T6SS machines found in Pseudomonas aeruginosa. This approach identified several putative toxin–immunity pairs, including Tse8–Tsi8. Full characterization of this protein pair demonstrated that Tse8 is delivered by the VgrG1a spike complex into prey cells where it targets the transamidosome, a multiprotein complex involved in protein synthesis in bacteria that lack either one, or both, of the asparagine and glutamine transfer RNA synthases. Biochemical characterization of the interactions between Tse8 and the transamidosome components GatA, GatB and GatC suggests that the presence of Tse8 alters the fine-tuned stoichiometry of the transamidosome complex, and in vivo assays demonstrate that Tse8 limits the ability of prey cells to synthesize proteins. These data expand the range of cellular components targeted by the T6SS by identifying a T6SS toxin affecting protein synthesis and validate the use of a transposon directed insertion site sequencing–based global genomics approach to expand the repertoire of T6SS toxins in T6SS-encoding bacteria.
AU  - Nolan,LM
AU  - Cain,AK
AU  - Clamens,T
AU  - Furniss,RCD
AU  - Manoli,E
AU  - Sainz-Polo,MA
AU  - Dougan,G
AU  - Albesa-Jove,D
AU  - Parkhill,J
AU  - Mavridou,DAI
AU  - Filloux,A
DO  - 10.1038/s41564-021-00950-8
EP  - 1199
PY  - 2021///
SN  - 2058-5276
SP  - 1199
TI  - Identification of tse8 as a type VI secretion system toxin from pseudomonas aeruginosa that targets the bacterial transamidosome to inhibit protein synthesis in prey cells
T2  - Nature Microbiology
UR  - http://dx.doi.org/10.1038/s41564-021-00950-8
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000686451200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41564-021-00950-8
UR  - http://hdl.handle.net/10044/1/97400
VL  - 6
ER  -
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