Publications
231 results found
Chan C, George AJT, Stark J, 2001, Cooperative enhancement of specificity in a lattice of T cell receptors, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 98, Pages: 5758-5763, ISSN: 0027-8424
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- Citations: 53
George AJ, 2001, Measurement of the kinetics of biomolecular interactions using the IAsys resonant mirror biosensor., Curr Protoc Immunol, Vol: Chapter 18, Pages: Unit-18.5
In this unit, the resonant mirror biosensor or IAsys, produced by Affinity Sensors, is discussed for the measurement of the kinetics of biomolecular interactions. All biosensors operate using a common principle--that binding events occurring on the sensing surface of the biosensor are converted by a transducer into an electronic signal which can then be processed by a computer to yield information on the amount of binding. Where biosensors vary is in their configuration and the nature of the transducer. Both the IAsys and BIACORE series of machines use optical techniques to detect the binding event, but the IAsys uses a resonant mirror device as the transducer, while the BIACORE uses surface plasmon resonance. When using biosensors the experiment can be divided into various phases, which are separately in this unit. The phases involve (1) the immobilization of the ligand onto the sensing surface; (2) addition of the ligate to the sensing surface, and following its association to the ligand, including the kinetics of binding; (3) washing of free ligate from chamber and following the dissociation of the ligand-ligate complex; (4) regeneration of the surface; repetition of phases 2 and 3; and (5) data analysis. A protocol is also included for optimization of ligand pH for immobilization to the carboxymethylated dextran surfaces described in this unit.
Lee L, George A, Buckley C, et al., 2001, Identification of novel adhesion ligands using peptide phage display: validation of the phage display library against α<sub>v</sub>β<sub>3</sub> integrin, Publisher: OXFORD UNIV PRESS, Pages: 67-67, ISSN: 1462-0324
Arancibia CV, George AJT, Ono SJ, et al., 2001, Expression of CIITA reconstitutes class II MHC expression on corneal endothelial cells: Implications for antigen presentation., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S471-S471, ISSN: 0146-0404
Keegan DJ, Ardjomand N, Halfyard AS, et al., 2001, Transplantation of retrovirus transfected engineered RPE cells expressing green fluorescent protein., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S781-S781, ISSN: 0146-0404
Tan PH, King WJ, Chen D, et al., 2001, Transferrin receptor-mediated gene transfer to the corneal endothelium, TRANSPLANTATION, Vol: 71, Pages: 552-560, ISSN: 0041-1337
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- Citations: 44
Griffith ME, Coulthart A, Pemberton S, et al., 2001, Anti-neutrophil cytoplasmic antibodies (ANCA) from patients with systemic vasculitis recognize restricted epitopes of proteinase 3 involving the catalytic site, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 123, Pages: 170-177, ISSN: 0009-9104
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- Citations: 46
Yates A, Chan CCW, Callard RE, et al., 2001, An approach to modelling in immunology, Briefings in Bioinformatics, Vol: 2, Pages: 245-257, ISSN: 1467-5463
George AJT, Larkin F, 2001, Corneal transplantation: prospects for gene therapy, Eye News, Vol: 7, Pages: 36-48, ISSN: 1368-8952
Huston JS, George AJ, 2001, Engineered antibodies take center stage., Hum Antibodies, Vol: 10, Pages: 127-142, ISSN: 1093-2607
The start of the post-genomic era provides a useful juncture for reflection on the state of antibody engineering, which will be a critical technology for relating function and pathology to genomic sequence in biology and medicine. The phenomenal progress in deciphering the human genome has given significant impetus to the application of engineered antibodies in proteomics. Thus, advances in phage display antibody libraries can now help to define novel gene function and the measurement of abnormal protein expression in pathological states. Furthermore, intrabody and antibody engineering provide vehicles for the development of molecular medicines of the future. In addition to these new directions, antibody engineering has begun to show concrete success in its long-term efforts to develop targeted immunotherapies for cancer and other diseases. The cornerstones of clinical development are the detailed academic clinical trials that continue to push the boundaries of engineered antibodies into the real world. The field displays a healthy impatience for practical results, as research accelerates with concerted efforts to transfer preclinical insights into clinical trials. Growing private and governmental expenditures will lead to the rapid expansion of life-saving immunotherapeutic agents. The present review developed from our effort to report on the 11th Annual International Conference on Antibody Engineering (3-6 December 2000). This annual meeting is a forum for discussions on the latest advances in antibody engineering groups from around the world, and now includes the broader agenda of engineering in molecular immunology. In bringing scientists together to exchange ideas at this open forum, new collaborations and the threads of new discoveries are woven. For example, Professors Gerhard Wagner (Harvard Medical School), Dennis Burton (Scripps Research Institute), and Peter Hudson (CSIRO, Melbourne, Australia) gave exciting insights on structural immunobiology that had i
King WJ, Comer RM, Hudde T, et al., 2000, Cytokine and chemokine expression kinetics after corneal transplantation, TRANSPLANTATION, Vol: 70, Pages: 1225-1233, ISSN: 0041-1337
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- Citations: 74
Rayner SA, King WJ, Comer RM, et al., 2000, Local bioactive tumour necrosis factor (TNF) in corneal allotransplantation, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 122, Pages: 109-116, ISSN: 0009-9104
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- Citations: 35
George AJT, Arancibia-Cárcamo CV, Awad HM, et al., 2000, Gene delivery to the corneal endothelium, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 162, Pages: S194-S200, ISSN: 1073-449X
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- Citations: 34
George AJT, Gray D, 2000, Jumping or walking: Which is better? [2], Immunology Today, Vol: 21, ISSN: 0167-5699
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- Citations: 1
Johns M, George AJT, Ritter MA, 2000, In vivo selection of sFv from phage display libraries, JOURNAL OF IMMUNOLOGICAL METHODS, Vol: 239, Pages: 137-151, ISSN: 0022-1759
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- Citations: 37
Hudde T, Rayner SA, De Alwis M, et al., 2000, Adeno-associated and herpes simplex viruses as vectors for gene transfer to the corneal endothelium, CORNEA, Vol: 19, Pages: 369-373, ISSN: 0277-3740
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- Citations: 45
Ramrakha PS, George A, Edwards R, et al., 2000, Intrabody mediated inhibition of VCAM expression on porcine endothelial cells as a strategy to prevent recruitment of human mononuclear leukocytes in xenotransplantation., TRANSPLANTATION, Vol: 69, Pages: S378-S378, ISSN: 0041-1337
George AJT, 2000, Peptide-mediated non-viral gene transfer for transplantation, TRANSPLANTATION, Vol: 69, Pages: 1031-1033, ISSN: 0041-1337
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- Citations: 4
Harris DJ, Larkin DFP, George AJT, et al., 2000, Overexpression of E2F-2 and its downstream effects on rabbit corneal endothelial cell proliferation in vitro., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S743-S743, ISSN: 0146-0404
Larkin DFP, Comer RM, King WJ, et al., 2000, Local and systemic CTLA-41g prolongs rat corneal allograft survival., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S543-S543, ISSN: 0146-0404
Arancibia CV, Haskova Z, George AJT, et al., 2000, Stressed corneal endothelium expresses class II MHC through a CHTA-independent pathway., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S667-S667, ISSN: 0146-0404
Tay E, King WJ, George AJT, et al., 2000, Constitutive and stimulated cytokine expression in rat corneas., Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, Pages: S670-S670, ISSN: 0146-0404
Midulla M, Verma R, Pignatelli M, et al., 2000, Source of oncofetal ED-B-containing fibronectin: Implications of production by both tumor and endothelial cells, CANCER RESEARCH, Vol: 60, Pages: 164-169, ISSN: 0008-5472
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- Citations: 61
George AJT, Gray D, 2000, Jumping or walking: which is better?, IMMUNOLOGY TODAY, Vol: 21, Pages: 56-56, ISSN: 0167-5699
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- Citations: 1
George AJT, 2000, The antibody molecule, Diagnostic and Therapeutic Antibodies, Editors: AJT, Urch, Totowa, NJ, Publisher: Humana Press, Pages: 1-21
Oral HB, George AJT, Haskard DO, 2000, Prevention of hydrogen peroxide- and cisplatin- induced apoptosis by intracellular catalase expression, Turkish Journal of Biology, Vol: 24, Pages: 685-696
George AJT, 2000, Use of biosensors to measure the kinetics of antibody-antigen interactions, Diagnostic and Therapeutic Antibodies, Editors: AJT, Urch, Totowa NJ, Publisher: Humana Press, Pages: 363-372
George AJT, Urch CE, 2000, Diagnostic and Therapeutic Antibodies, Totowa, New Jersey, Publisher: Humana Press, ISBN: 9780896037984
Sepp A, Farrar CA, Dorling T, et al., 1999, Inhibition of expression of the Galα1-3Gal epitope on porcine cells using an intracellular single-chain antibody directed against α1,3Galactosyltransferase, JOURNAL OF IMMUNOLOGICAL METHODS, Vol: 231, Pages: 191-205, ISSN: 0022-1759
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- Citations: 5
George AJT, 1999, Antibody engineering: an introduction, JOURNAL OF IMMUNOLOGICAL METHODS, Vol: 231, Pages: 1-1, ISSN: 0022-1759
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