Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sumrall:2019:10.1371/journal.ppat.1008032,
author = {Sumrall, ET and Shen, Y and Keller, AP and Rismondo, J and Pavlou, M and Eugster, MR and Boulos, S and Disson, O and Thouvenot, P and Kilcher, S and Wollscheid, B and Cabanes, D and Lecuit, M and Gründling, A and Loessner, MJ},
doi = {10.1371/journal.ppat.1008032},
journal = {PLoS Pathogens},
title = {Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion},
url = {http://dx.doi.org/10.1371/journal.ppat.1008032},
volume = {15},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells. Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks. The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns. Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen. We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d. Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo. We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness. Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB. Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.
AU  - Sumrall,ET
AU  - Shen,Y
AU  - Keller,AP
AU  - Rismondo,J
AU  - Pavlou,M
AU  - Eugster,MR
AU  - Boulos,S
AU  - Disson,O
AU  - Thouvenot,P
AU  - Kilcher,S
AU  - Wollscheid,B
AU  - Cabanes,D
AU  - Lecuit,M
AU  - Gründling,A
AU  - Loessner,MJ
DO  - 10.1371/journal.ppat.1008032
PY  - 2019///
SN  - 1553-7366
TI  - Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1008032
UR  - http://hdl.handle.net/10044/1/74039
VL  - 15
ER  -
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