Imperial College London
Alternate

Professor Angelika Gründling

Faculty of Medicine, Department of Infectious Disease

Professor of Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 5256a.grundling Website

 
 
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Location

 

6.22Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Schuster:2020:10.1111/mmi.14433,
author = {Schuster, CF and Wiedemann, DM and Kirsebom, FCM and Santiago, M and Walker, S and Gründling, A},
doi = {10.1111/mmi.14433},
journal = {Molecular Microbiology},
pages = {699--717},
title = {Highthroughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors},
url = {http://dx.doi.org/10.1111/mmi.14433},
volume = {113},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Staphylococcus aureus is an opportunistic pathogen that can cause soft tissue infections but is also a frequent cause of foodborne illnesses. One contributing factor for this food association is its high salt tolerance allowing this organism to survive commonly used food preservation methods. How this resistance is mediated is poorly understood, particularly during longterm exposure. In this study, we used TNseq to understand how the responses to osmotic stressors differ. Our results revealed distinctly different longterm responses to NaCl, KCl and sucrose stresses. In addition, we identified the DUF2538 domain containing gene SAUSA300_0957 (gene 957) as essential under salt stress. Interestingly, a 957 mutant was less susceptible to oxacillin and showed increased peptidoglycan crosslinking. The salt sensitivity phenotype could be suppressed by amino acid substitutions in the transglycosylase domain of the penicillin binding protein Pbp2, and these changes restored the peptidoglycan crosslinking to WT levels. These results indicate that increased crosslinking of the peptidoglycan polymer can be detrimental and highlight a critical role of the bacterial cell wall for osmotic stress resistance. This study will serve as a starting point for future research on osmotic stress response and help develop better strategies to tackle foodborne staphylococcal infections.
AU  - Schuster,CF
AU  - Wiedemann,DM
AU  - Kirsebom,FCM
AU  - Santiago,M
AU  - Walker,S
AU  - Gründling,A
DO  - 10.1111/mmi.14433
EP  - 717
PY  - 2020///
SN  - 0950-382X
SP  - 699
TI  - Highthroughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors
T2  - Molecular Microbiology
UR  - http://dx.doi.org/10.1111/mmi.14433
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14433
UR  - http://hdl.handle.net/10044/1/75249
VL  - 113
ER  -
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