Imperial College London
Alternate

Professor Amin Hajitou

Faculty of Medicine, Department of Brain Sciences

Professor of Targeted Therapeutics
 
 
 
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Contact

 

+44 (0)20 7594 6546a.hajitou Website

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Namdee:2018:10.1016/j.omtn.2018.04.012,
author = {Namdee, K and Khongkow, M and Boonrungsiman, S and Nittayasut, N and Asavarut, P and Temisak, S and Saengkrit, N and Puttipipatkhachorn, S and Hajitou, A and Ruxrungtham, K and Yata, T},
doi = {10.1016/j.omtn.2018.04.012},
journal = {Molecular Therapy : Nucleic Acids},
pages = {33--44},
title = {Thermoresponsive bacteriophage nanocarrier as a gene delivery vector targeted to the gastrointestinal tract.},
url = {http://dx.doi.org/10.1016/j.omtn.2018.04.012},
volume = {12},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The use of the gastrointestinal tract as a site for the local delivery of DNA is an exciting prospect. In order to obtain an effective vector capable of delivering a gene of interest to target cells to achieve sufficient and sustained transgene expression, with minimal toxicity, we developed a new generation of filamentous bacteriophage. This particular bacteriophage was genetically engineered to display an arginine-glycine-aspartic acid (RGD) motif (an integrin-binding peptide) on the major coat protein pVIII and carry a mammalian DNA cassette. One unanticipated observation is the thermoresponsive behavior of engineered bacteriophage. This finding has led us to simplify the isolation method to purify bacteriophage particles from cell culture supernatant by low-temperature precipitation. Our results showed that, in contrast to non-surface modified, the RGD-modified bacteriophage was successfully used to deliver a transgene to mammalian cells. Our in vitro model of the human intestinal follicle-associated epithelium also demonstrated that bacteriophage particles were stable in simulated gastrointestinal fluids and able to cross the human intestinal barrier. In addition, we confirmed an adjuvant property of the engineered bacteriophage to induce nitric oxide production by macrophages. In conclusion, our study demonstrated the possibility of using bacteriophage for gene transfer in the gastrointestinal tract.
AU  - Namdee,K
AU  - Khongkow,M
AU  - Boonrungsiman,S
AU  - Nittayasut,N
AU  - Asavarut,P
AU  - Temisak,S
AU  - Saengkrit,N
AU  - Puttipipatkhachorn,S
AU  - Hajitou,A
AU  - Ruxrungtham,K
AU  - Yata,T
DO  - 10.1016/j.omtn.2018.04.012
EP  - 44
PY  - 2018///
SN  - 2162-2531
SP  - 33
TI  - Thermoresponsive bacteriophage nanocarrier as a gene delivery vector targeted to the gastrointestinal tract.
T2  - Molecular Therapy : Nucleic Acids
UR  - http://dx.doi.org/10.1016/j.omtn.2018.04.012
UR  - http://hdl.handle.net/10044/1/60014
VL  - 12
ER  -
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