Publications

BibTex format

@article{Dobroff:2016:10.1073/pnas.1615288113,
author = {Dobroff, AS and D'Angelo, S and Eckhardt, BL and Ferrara, F and Staquicini, DI and Cardo-Vila, M and Staquicini, FI and Nunes, DN and Kim, K and Driessen, WHP and Hajitou, A and Lomo, LC and Barry, M and Krishnamurthy, S and Sahin, A and Woodward, WA and Prossnitz, ER and Anderson, RL and Dias-Neto, E and Brown-Glaberman, UA and Royce, ME and Ueno, NT and Cristofanilli, M and Hortobagyi, GN and Marchio, S and Gelovani, JG and Sidman, RL and Arap, W and Pasqualini, R},
doi = {10.1073/pnas.1615288113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
pages = {12780--12785},
title = {Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes},
url = {http://dx.doi.org/10.1073/pnas.1615288113},
volume = {113},
year = {2016}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Inflammatory breast carcinoma (IBC) is one of the most lethal forms of human breast cancer, and effective treatment for IBC is an unmet clinical need in contemporary oncology. Tumor-targeted theranostic approaches are emerging in precision medicine, but only a few specific biomarkers are available. Here we report up-regulation of the 78-kDa glucose-regulated protein (GRP78) in two independent discovery and validation sets of specimens derived from IBC patients, suggesting translational promise for clinical applications. We show that a GRP78-binding motif displayed on either bacteriophage or adeno-associated virus/phage (AAVP) particles or loop-grafted onto a human antibody fragment specifically targets orthotopic IBC and other aggressive breast cancer models in vivo. To evaluate the theranostic value, we used GRP78-targeting AAVP particles to deliver the human Herpes simplex virus thymidine kinase type-1 (HSVtk) transgene, obtaining simultaneous in vivo diagnosis through PET imaging and tumor treatment by selective activation of the prodrug ganciclovir at tumor sites. Translation of this AAVP system is expected simultaneously to image, monitor, and treat the IBC phenotype and possibly other aggressive (e.g., invasive and/or metastatic) subtypes of breast cancer, based on the inducible cell-surface expression of the stress-response chaperone GRP78, and possibily other cell-surface receptors in human tumors.
AU  - Dobroff,AS
AU  - D'Angelo,S
AU  - Eckhardt,BL
AU  - Ferrara,F
AU  - Staquicini,DI
AU  - Cardo-Vila,M
AU  - Staquicini,FI
AU  - Nunes,DN
AU  - Kim,K
AU  - Driessen,WHP
AU  - Hajitou,A
AU  - Lomo,LC
AU  - Barry,M
AU  - Krishnamurthy,S
AU  - Sahin,A
AU  - Woodward,WA
AU  - Prossnitz,ER
AU  - Anderson,RL
AU  - Dias-Neto,E
AU  - Brown-Glaberman,UA
AU  - Royce,ME
AU  - Ueno,NT
AU  - Cristofanilli,M
AU  - Hortobagyi,GN
AU  - Marchio,S
AU  - Gelovani,JG
AU  - Sidman,RL
AU  - Arap,W
AU  - Pasqualini,R
DO  - 10.1073/pnas.1615288113
EP  - 12785
PY  - 2016///
SN  - 0027-8424
SP  - 12780
TI  - Towards a transcriptome-based theranostic platform for unfavorable breast cancer phenotypes
T2  - Proceedings of the National Academy of Sciences of the United States of America
UR  - http://dx.doi.org/10.1073/pnas.1615288113
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000388073300069&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/57868
VL  - 113
ER  -

Download

Professor Amin Hajitou

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)