Imperial College London
Alternate

Dr Andrei S. Kozlov

Faculty of Engineering, Department of Bioengineering

Reader in Sensory Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 1338a.kozlov Website

 
 
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Location

 

RSM 3.12Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sirko:2022:10.12688/wellcomeopenres.17428.1,
author = {Sirko, P and Kozlov, AS},
doi = {10.12688/wellcomeopenres.17428.1},
journal = {Wellcome Open Research},
pages = {1--1},
title = {Immunohistochemistry localises myosin-7a to cochlear efferent boutons},
url = {http://dx.doi.org/10.12688/wellcomeopenres.17428.1},
volume = {7},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <ns4:p><ns4:bold>Background: </ns4:bold>Myosin 7a is an actin-binding motor protein involved in the formation of hair-cell stereocilia both in the cochlea and in the vestibular system. Mutations in myosin 7a are linked to congenital hearing loss and are present in 50% of Type-1 Usher syndrome patients who suffer from progressive hearing loss and vestibular system dysfunction.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>Myosin 7a is often used to visualise sensory hair cells due to its well characterised and localised expression profile. We thus conducted myosin-7a immunostaining across all three turns of the adult rat organ of Corti to visualise hair cells.</ns4:p><ns4:p> <ns4:bold>Results: </ns4:bold>As expected, we observed myosin 7a staining in both inner and outer hair cells. Unexpectedly, we also observed strong myosin 7a staining in the medial olivocochlear efferent synaptic boutons contacting the outer hair cells. Efferent bouton myosin-7a staining was present across all three turns of the cochlea. We verified this localisation by co-staining with a known efferent bouton marker, the vesicular acetylcholine transporter.</ns4:p><ns4:p> <ns4:bold>Conclusions: </ns4:bold>In addition to its role in stereocilia formation and maintenance, myosin 7a or certain myosin-7a expression variants might play a role in efferent synaptic transmission in the cochlea and thus ultimately influence cochlear gain regulation. Our immunohistochemistry results should be validated with other methods to confirm these serendipitous findings.</ns4:p>
AU  - Sirko,P
AU  - Kozlov,AS
DO  - 10.12688/wellcomeopenres.17428.1
EP  - 1
PY  - 2022///
SP  - 1
TI  - Immunohistochemistry localises myosin-7a to cochlear efferent boutons
T2  - Wellcome Open Research
UR  - http://dx.doi.org/10.12688/wellcomeopenres.17428.1
VL  - 7
ER  -
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