Imperial College London
Portrait Picture

PROFESSOR AJIT LALVANI

Faculty of Medicine, National Heart & Lung Institute

Chair in Infectious Diseases
 
 
 
//

Contact

 

+44 (0)20 7594 0883a.lalvani

 
 
//

Assistant

 

Dr Luis Berrocal Almanza +44 (0)20 7594 3721

 
//

Location

 

Medical SchoolSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Shaikh:2017:10.5588/ijtld.16.0428,
author = {Shaikh, N and Gupte, A and Dharmshale, S and Pokkali, S and Thakar, M and Upadhye, VJ and Ordonez, AA and Kinikar, A and Gupte, N and Mave, V and Kagal, A and Gupta, A and Lalvani, A and Paranjpe, R and Bharadwaj, R and Jain, SK},
doi = {10.5588/ijtld.16.0428},
journal = {Int J Tuberc Lung Dis},
pages = {412--419},
title = {Novel interferon-gamma assays for diagnosing tuberculosis in young children in India.},
url = {http://dx.doi.org/10.5588/ijtld.16.0428},
volume = {21},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - SETTING: The tuberculin skin test (TST) and interferon-gamma release assays (IGRAs) are used as supportive evidence to diagnose active tuberculosis (TB). Novel IGRAs could improve diagnosis, but data are lacking in young children. DESIGN: Children (age 5 years) with suspected TB were prospectively screened at a tertiary hospital in Pune, India; the children underwent TST, and standard (early secretory antigenic target 6 and culture filtrate protein 10) and enhanced (five additional novel antigens) enzyme-linked immunospot (ELISpot) assays. RESULTS: Of 313 children (median age 30 months) enrolled, 92% had received bacille Calmette-Guérin vaccination, 53% were malnourished and 9% were coinfected with the human immunodeficiency virus (HIV); 48 (15%) had TB, 128 (41%) did not, and TB could not be ruled out in 137 (44%). The sensitivity of enhanced (45%) and standard (42%) ELISpot assays for diagnosing TB was better than that of TST (20%) (P  0.03); however, enhanced ELISpot was not more sensitive than the standard ELISpot assay (P = 0.50). The specificity of enhanced ELISpot, standard ELISpot and TST was respectively 82% (95%CI 74-89), 88% (95%CI 81-94) and 98% (95%CI 93-100). Rv3879c and Rv3615c, previously reported to be promising antigens, failed to improve the diagnostic performance of the ELISpot assay. CONCLUSION: The TST and the standard and novel ELISpot assays performed poorly in diagnosing active TB among young children in India.
AU  - Shaikh,N
AU  - Gupte,A
AU  - Dharmshale,S
AU  - Pokkali,S
AU  - Thakar,M
AU  - Upadhye,VJ
AU  - Ordonez,AA
AU  - Kinikar,A
AU  - Gupte,N
AU  - Mave,V
AU  - Kagal,A
AU  - Gupta,A
AU  - Lalvani,A
AU  - Paranjpe,R
AU  - Bharadwaj,R
AU  - Jain,SK
DO  - 10.5588/ijtld.16.0428
EP  - 419
PY  - 2017///
SP  - 412
TI  - Novel interferon-gamma assays for diagnosing tuberculosis in young children in India.
T2  - Int J Tuberc Lung Dis
UR  - http://dx.doi.org/10.5588/ijtld.16.0428
UR  - https://www.ncbi.nlm.nih.gov/pubmed/28284256
VL  - 21
ER  -
Download