Evo-devo using nematodes
My research mostly concerns the genetic control of growth in the nematode Caenorhabditis elegans. This is the minute worm which formed the basis of the studies for which Sulston, Horvitz and Brenner won the Nobel Prize in 2002. We are particularly interested in how several molecular signalling pathways control the size to which the worm grows (about 1 mm long). We are studying the TGF-beta (DBL-1) pathway, a molecule which is secreted by the worm's ventral nerve cord and appears to act rather like growth hormone. We are investigating the genes that are the target of this pathway, how it influences the cells of growing worms, the size differences between the sexes (hermaphrodite worms are larger than males), and the way in which it mediates signals from the environment. We are also interested in another, quite different, growth control system that we identified in 2002: one that emanates from the worm's gonad. To study these problems we use a variety of standard molecular techniques, including screening for gigantism and dwarfism mutants, and some that are more unique to worms such as laser microsurgery.
Our research is not, however, merely motivated by understanding growth in C. elegans. We also want to understand the genetic basis of body size evolution in nematodes. We have a zoo of about 50 species of free-living nematodes that we keep in the laboratory much as we keep C. elegans (on petri dishes eating E. coli), some of which are larger than C. elegans itself. We believe that the TGF-beta pathway is involved in these evolutionary differences.
We are also interested in computational models of nematode development. Using software developed by collaborators at DKFZ, Heidelberg and Houston, we are analysing the lineage and three-dimensional topology of embryonic development.
The metabolic basis of growth and longevity
A great deal is known about growth and ageing in C. elegans: many genes have identified that alter either the size or the longevity of the worm. But very little is known about how these genes actually work together to control these attributes. For example, they must all affect the worm's metabolism – but how? To answer this question, we have been using "metabolic profiling" – a technique for assaying the concentrations of hundreds of metabolites simultaneously in tissues. Our collaborator is Jake Bundy from SORA, Imperial College; he's an expert in the technique. We expect that our studies will add an important new experimental approach to functional genetics in animals.
The evolution of music
All humans sing. But they don't usually just make songs up; they learn them from other people. This means that song styles evolve rather as languages do. Inspired by the early work of Alan Lomax, a pioneer of ethnomusicology - the study of non-Western music - we are attempting to use evolutionary algorithms to trace out the history of song styles. We are analysing thousands of songs from around the world to find out how they are related to each other. Perhaps we can even identify some of the features of the very first song sung by humans. Our collaborators on this project are the musician Brian Eno and the Alan Lomax Foundation in New York.
- 2002-2005 BBSRC Project Grant,
Molecular basis of body size evolution in nematodes
- 2002-2005 BBSRC Project Grant,
Identifying targets of TGF-beta signalling in C. elegans
- 1997-2001 BBSRC Project Grant
Cellular basis of body size evolution in nematodes
Invited Lectures and Presentations
- 11.2007 - Merck, Sharpe & Dohme Seminar, Conway Institute, Dublin
- 11.2007 - LERN Lecture, Natural History Museum, London
- 04.2007 - SCAMPS Lecture, Sanger Centre, Cambridge
- 11. 2004 - Pfizer Annual Lecture. Natural History Museum, London
- 08. 2004 - Royal Institution of Great Britain
Dr Jake Bundy, Surgery and Cancer, Imperial College London
Professor Austin Burt, Life Sciences, Imperial College London