Imperial College London
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Dr Alex Miras

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)7958 377 674a.miras

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Swan:2022:10.1089/dia.2022.2525.abstracts,
author = {Swan, P and Johnson, B and Samarasinghe, S and De, Vito G and Cowley, M and Le, Roux C and Miras, A and Docherty, N},
doi = {10.1089/dia.2022.2525.abstracts},
pages = {A44--A44},
publisher = {Mary Ann Liebert},
title = {Alpha-melanocyte stimulatory hormone: a novel player in post-prandial glucose disposal in skeletal muscle in humans},
url = {http://dx.doi.org/10.1089/dia.2022.2525.abstracts},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - Background and Aims: Studies in rodents demonstrate that increases in circulating pituitaryderived alphamelanocyte stimulatory hormone (αMSH) contribute to postprandial glycaemic control. Moreover, intravenous administration of exogenous αMSH lowers glucose excursions during oral glucose tolerance testing (OGTT) in mice. We set out to interrogate whether this action translated to human physiology both in vivo and in vitroMethods: Using a randomized doubleblinded crossover design, fifteen healthy volunteers received infusions of physiological saline, 15, 150 and 1500ng/kg/hr αMSH initiated 30 minutes prior to the administration of a standard OGTT. Plasma glucose and insulin was measured during the OGTT. To assess the effect of αMSH on glucose disposal into skeletal muscle disposal, 15 subjects underwent sequential hyperinsulinaemiceuglycaemic clamp, concomitant to either saline or 150ng/kg/hr αMSH infusion. In a separate cohort of healthy volunteers (n=6), vastus lateralis muscle biopsies were obtained and used to establish cultures of primary human myotubes. Tritiated 2deoxyDglucose was used to monitor glucose uptake in response to αMSH.Results: Infusion of αMSH (1500ng/kg/hr) reduced the incremental area under the curve (iAUC) for plasma glucose (p=0.02), and plasma insulin (p=0.006) by approximately 20%. At high steady state insulin concentrations in clamp studies, αMSH increased glucose requirements for the maintenance of euglycaemia. Primary human myotube cultures expressed melanocortin receptor subtypes (MC1R>MC3R≈MC4R) and both 10nM and 100nM αMSH increased glucose uptake by twofold versus vehicle (p=0.001).Conclusions: These findings substantiate a role for peripheral αMSH as a hitherto undescribed component of the endocrine control of glycaemia in human physiology.
AU  - Swan,P
AU  - Johnson,B
AU  - Samarasinghe,S
AU  - De,Vito G
AU  - Cowley,M
AU  - Le,Roux C
AU  - Miras,A
AU  - Docherty,N
DO  - 10.1089/dia.2022.2525.abstracts
EP  - 44
PB  - Mary Ann Liebert
PY  - 2022///
SN  - 1520-9156
SP  - 44
TI  - Alpha-melanocyte stimulatory hormone: a novel player in post-prandial glucose disposal in skeletal muscle in humans
UR  - http://dx.doi.org/10.1089/dia.2022.2525.abstracts
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000791212200119&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.liebertpub.com/doi/10.1089/dia.2022.2525.abstracts
UR  - http://hdl.handle.net/10044/1/98141
ER  -
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