Imperial College London
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DrAnitaMitra

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Lecturer
 
 
 
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a.mitra

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mitra:2017:10.1038/s41598-017-09844-4,
author = {Mitra, A and Mahajan, V and Macintyre, D and Lee, Y and Smith, A and Marchesi, J and Lyons, D and Bennett, P and Kyrgiou, M},
doi = {10.1038/s41598-017-09844-4},
journal = {Scientific Reports},
title = {Comparison of vaginal microbiota sampling techniques: cytobrush versus swab},
url = {http://dx.doi.org/10.1038/s41598-017-09844-4},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Evidence suggests the vaginal microbiota (VM) may influence risk of persistent Human Papillomavirus (HPV) infection and cervical carcinogenesis. Established cytology biobanks, typically collected with a cytobrush, constitute a unique resource to study such associations longitudinally. It is plausible that compared to rayon swabs; the most commonly used sampling devices, cytobrushes may disrupt biofilms leading to variation in VM composition. Cervico-vaginal samples were collected with cytobrush and rayon swabs from 30 women with high-grade cervical precancer. Quantitative PCR was used to compare bacterial load and Illumina MiSeq sequencing of the V1-V3 regions of the 16S rRNA gene used to compare VM composition. Cytobrushes collected a higher total bacterial load. Relative abundance of bacterial species was highly comparable between sampling devices (R2 = 0.993). However, in women with a Lactobacillus-depleted, high-diversity VM, significantly less correlation in relative species abundance was observed between devices when compared to those with a Lactobacillus species-dominant VM (p = 0.0049). Cytobrush and swab sampling provide a comparable VM composition. In a small proportion of cases the cytobrush was able to detect underlying high-diversity community structure, not realized with swab sampling. This study highlights the need to consider sampling devices as potential confounders when comparing multiple studies and datasets.
AU  - Mitra,A
AU  - Mahajan,V
AU  - Macintyre,D
AU  - Lee,Y
AU  - Smith,A
AU  - Marchesi,J
AU  - Lyons,D
AU  - Bennett,P
AU  - Kyrgiou,M
DO  - 10.1038/s41598-017-09844-4
PY  - 2017///
SN  - 2045-2322
TI  - Comparison of vaginal microbiota sampling techniques: cytobrush versus swab
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-017-09844-4
UR  - http://hdl.handle.net/10044/1/50313
VL  - 7
ER  -
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