Publications
124 results found
Newman Taylor AJ, 2001, Role of human leukocyte antigen phenotype and exposure in development of occupational asthma, Curr Opin Allergy Clin Immunol, Vol: 1, Pages: 157-161, ISSN: 1528-4050
Cullinan P, Harris JM, Taylor AJN, et al., 2000, An outbreak of asthma in a modern detergent factory, LANCET, Vol: 356, Pages: 1899-1900, ISSN: 0140-6736
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- Citations: 111
Barker RD, van Tongeren MJA, Harris JM, et al., 2000, Risk factors for bronchial hyperresponsiveness in workers exposed to acid anhydrides, EUROPEAN RESPIRATORY JOURNAL, Vol: 15, Pages: 710-715, ISSN: 0903-1936
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- Citations: 14
Jones MG, Welch J, Cullinan P, et al., 2000, Allergenicity of grass and oil seed rape pollen, IMMUNOLOGY TODAY, Vol: 21, Pages: 155-155, ISSN: 0167-5699
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- Citations: 1
Welch J, Jones MG, Cullinan P, et al., 2000, Sensitization to oilseed rape is not due to cross-reactivity with grass pollen, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 30, Pages: 370-375, ISSN: 0954-7894
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- Citations: 14
Selassie FG, Stevens RH, Cullinan P, et al., 2000, Total and specific IgE (house dust mite and intestinal helminths) in asthmatics and controls from Gondar, Ethiopia, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 30, Pages: 356-358, ISSN: 0954-7894
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- Citations: 38
Taylor AJN, Cullinan P, Lympany PA, et al., 1999, Interaction of HLA phenotype and exposure intensity in sensitization to complex platinum salts, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 160, Pages: 435-438, ISSN: 1073-449X
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- Citations: 63
Cullinan P, Cook A, Gordon S, et al., 1999, Allergen exposure, atopy and smoking as determinants of allergy to rats in a cohort of laboratory employees, EUROPEAN RESPIRATORY JOURNAL, Vol: 13, Pages: 1139-1143, ISSN: 0903-1936
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- Citations: 123
Nieuwenhuijsen MJ, Heederik D, Doekes G, et al., 1999, Exposure-response relations of α-amylase sensitisation in British bakeries and flour mills, OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, Vol: 56, Pages: 197-201, ISSN: 1351-0711
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- Citations: 63
Atkinson W, Harris J, Mills P, et al., 1999, Domestic aeroallergen exposures among infants in an English town, EUROPEAN RESPIRATORY JOURNAL, Vol: 13, Pages: 583-589, ISSN: 0903-1936
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- Citations: 41
Nightingale JA, Cullinan P, Ashmore M, et al., 1999, Inflammatory effects of inhaled diesel exhaust particulates in normal subjects., AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 159, Pages: A317-A317, ISSN: 1073-449X
van Tongeren MJA, Barker RD, Gardiner K, et al., 1998, Retrospective exposure assessment for a cohort study into respiratory effects of acid anhydrides, OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, Vol: 55, Pages: 692-696, ISSN: 1351-0711
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- Citations: 8
Barker RD, van Tongeren MJA, Harris JM, et al., 1998, Risk factors for sensitisation and respiratory symptoms among workers exposed to acid anhydrides: a cohort study, OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, Vol: 55, Pages: 684-691, ISSN: 1351-0711
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- Citations: 60
Adisesh LA, Kharitonov SA, Yates DH, et al., 1998, Exhaled and nasal nitric oxide is increased in laboratory animal allergy, CLINICAL AND EXPERIMENTAL ALLERGY, Vol: 28, Pages: 876-880, ISSN: 0954-7894
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- Citations: 46
Barker RD, Harris JM, Welch JA, et al., 1998, Occupational asthma caused by tetrachlorophthalic anhydride: A 12-year follow-up, JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol: 101, Pages: 717-719, ISSN: 0091-6749
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- Citations: 18
Tee RD, Cullinan P, Welch J, et al., 1998, Specific IgE to isocyanates: A useful diagnostic role in occupational asthma, JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol: 101, Pages: 709-715, ISSN: 0091-6749
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- Citations: 135
Renström A, Gordon S, Larsson PH, et al., 1997, Comparison of a radioallergosorbent (RAST) inhibition method and a monoclonal enzyme linked immunosorbent assay (ELISA) for aeroallergen measurement., Clin Exp Allergy, Vol: 27, Pages: 1314-1321, ISSN: 0954-7894
BACKGROUND: Mouse and rat urinary proteins are potent occupational allergens for exposed personnel. Methods of measuring airborne allergens differ greatly, and reported levels of allergens vary considerably between laboratories. OBJECTIVES: To compare the values obtained using two different methods of allergen detection. METHODS: Air samples were collected in rat rooms in Sweden and the United Kingdom at 2 L/min on to polytetrafluoroethylene (PTFE) filters and extracted in buffer containing 0.5% v/v Tween 20. Airborne rat urinary allergen (RUA) was measured in all samples by both RAST inhibition using a polyclonal human serum pool (UK) and a two monoclonal antibody sandwich ELISA employing antibodies specific for Rat n 1.02 (alpha2u-globulin) (Sweden). RESULTS: The two methods gave values which were correlated (r2 log values = 0.72, P<0.0001), but differed by several orders of magnitude (median [range] ratio of RAST inhibition/ELISA = 316 [7-26(80)]. There was a systematic bias: as the absolute values increased, the difference in the measurements increased. The rat urine standards used were antigenically similar. CONCLUSIONS: A large contrast in RUA values obtained from the two assays was observed in this study. This may be primarily due to methodological differences, but variations in antibody specificities or composition of allergenic epitopes in the air samples may contribute. The results demonstrate that standardization of methods and antibodies is necessary before interlaboratory comparisons can be made.
Sandiford CP, Tatham AS, Fido R, et al., 1997, Identification of the major water/salt insoluble wheat proteins involved in cereal hypersensitivity., Clin Exp Allergy, Vol: 27, Pages: 1120-1129, ISSN: 0954-7894
BACKGROUND: Several studies have investigated water/salt soluble proteins which comprise 50% of the proteins in wheat. The remaining 50% of wheat proteins, are water/salt insoluble proteins of which there is limited information on their role in cereal hypersensitivity. OBJECTIVES: To investigate the allergenicity of the water/salt insoluble gliadin and glutenin proteins (prolamins). METHODS: RAST, electrophoresis and Western blotting were used to identify water/salt insoluble wheat allergens. Competitive RAST inhibition was conducted to investigate cross-reactivity between prolamins and water/salt soluble wheat proteins. RESULTS: Specific IgE to alpha-gliadin and to total glutenins were detected in all sera. IgE to beta-, gamma-, fast omega-, and slow omega-gliadin were present in lower numbers of sera. Prolamin allergens of 90-11 kDa were identified by immunoblotting. Water/salt soluble proteins crossreacted with alpha-gliadin and total glutenins. CONCLUSIONS: Individuals who are hypersensitive to water/salt soluble wheat proteins produce specific IgE to water/salt insoluble wheat proteins. Western blotting has shown that gliadins, glutenins and proteins with similar molecular weights as the endogenous water/salt soluble wheat enzyme inhibitors are important allergens. Alpha and fast omega- are the most allergenic gliadins. The water/salt insoluble proteins share cross-reacting epitopes with water/salt soluble proteins. These data show that the numbers of proteins involved in the development of cereal hypersensitivity is greater than previously believed and that the development of specific IgE to alpha-gliadin may in part depend on the presence of cross-reacting antibodies to water/salt soluble flour allergens.
Gordon S, Wallace J, Cook A, et al., 1997, Reduction of exposure to laboratory animal allergens in the workplace., Clin Exp Allergy, Vol: 27, Pages: 744-751, ISSN: 0954-7894
OBJECTIVES: Evidence is now accumulating that the prevalence of allergy to laboratory animals is related to the intensity of exposure to animal allergens. Whilst airborne animal allergen concentrations may be influenced by the litter type, cage design and stock density, the effectiveness of methods to reduce personal exposure has not been objectively assessed. METHODS: Air samples were collected at 2 L/min and 180 L/min onto polytetrafluoroethylene (PTFE) filters and the rat urinary aeroallergen (RUA) and mouse urinary aeroallergen (MUA) concentrations were measured by radioallergosorbent test (RAST) inhibition. RESULTS: When 545 mice (11.l mice/m3) were housed in ventilated cages (Thoren Maximiser cage system) operated at positive pressure to the environment, the static MUA concentration (n = 24, median = 0.10 microg/m3) was reduced sevenfold when compared with conventional cage systems (n = 12, median =0.67 microg/m3, P< 0.001). MUA could be further reduced if the ventilated cage system was operated at lower pressure; static samples (n = 1) collected at 180 L/min at negative, ambient and positive pressure registered < 0.003, 0.02 and 0.28 microg/m3, respectively. During cleaning out, the intensity of personal exposure to RUA was apparently reduced twofold when soiled litter was removed by vacuum (n = 17, median = 22.87 microg/m3) when compared with tipping (n = 18, median = 38.15 microg/m3), P = 0.002) although the task took twice as long to perform. The RUA exposure associated with handling rats was'reduced 25-fold when performed in a ventilated cabinet (n = 21, median = 2.67 microg/m3) compared with handling of rats on an open bench (n = 17, median = 54.39 microg/m3, P = 0.0001). CONCLUSIONS: Effective reduction of exposure to animal allergens can now be achieved by the use of ventilated systems both for housing and handling rats and mice providing safety equipment is used correctly. The vacuum removal of soiled litter during the task of cleaning out was l
Gordon S, Jones MG, Tee RD, et al., 1997, Review: the biochemistry of common aeroallergens. C. A. Stewart and P. J. Thompson, September 1996; 26:1020-44., Clin Exp Allergy, Vol: 27, Pages: 714-715, ISSN: 0954-7894
Cullinan P, Newman Taylor AJ, 1997, Aetiology of occupational asthma., Clin Exp Allergy, Vol: 27 Suppl 1, Pages: 41-46, ISSN: 0954-7894
Elliott MW, Newman Taylor AJ, 1997, Allergic bronchopulmonary aspergillosis., Clin Exp Allergy, Vol: 27 Suppl 1, Pages: 55-59, ISSN: 0954-7894
Cullinan P, Cook A, Jones M, et al., 1997, Clinical responses to ingested fungal alpha-amylase and hemicellulase in persons sensitized to Aspergillus fumigatus?, ALLERGY, Vol: 52, Pages: 346-349, ISSN: 0105-4538
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- Citations: 100
Gordon S, Kiernan LA, Nieuwenhuijsen MJ, et al., 1997, Measurement of exposure to mouse urinary proteins in an epidemiological study, OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, Vol: 54, Pages: 135-140, ISSN: 1351-0711
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- Citations: 20
Cullinan P, Taylor AJN, 1997, Inferences from occupational asthma, RISING TRENDS IN ASTHMA, Vol: 206, Pages: 160-172, ISSN: 0300-5208
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- Citations: 5
Lympany PA, Petrek M, Southcott AM, et al., 1996, HLA-DPB polymorphisms: Glu 69 association with sarcoidosis., Eur J Immunogenet, Vol: 23, Pages: 353-359, ISSN: 0960-7420
Sarcoidosis is a chronic granulomatous disorder, which is characterized by the accumulation of activated CD4+ T lymphocytes (T cells) at disease sites. There is up-regulation of cell surface expression of MHC molecules in sarcoidosis, and it has been suggested that specific MHC class II alleles are associated with the disease. A study of chronic beryllium disease (CBD), a granulomatous disorder which is pathologically similar to sarcoidosis, has identified an association between this disease and the presence of a glutamine residue at position 69 (Glu 69+) of the B1 chain of the HLA-DPB molecule. A further study also suggested the importance of Glu at position 55 of the same chain. The aims of the present study were to attempt to define MHC class II alleles associated with sarcoidosis by comparison of their frequency in two groups of subjects and to compare the frequency of HLA-DPB1 Glu 69+/- and Glu 55+/-alleles in the same subjects. Forty-one subjects with sarcoidosis and 76 normal subjects were studied. The polymorphic regions of the class II MHC were identified by PCR in association with sequence-specific oligonucleotide probes. There were no significant differences in the phenotype frequencies of MHC class II or Glu 55+ alleles between the two groups of subjects. However, there was a significant increase (P = 0.02) in the frequency of HLA-DPB1* Glu 69+ alleles compared with the control population. We therefore suggest that the presence of a Glu residue at position 69 on the DPB1 chain may play an important role in antigen presentation and recognition in chronic granulomatous diseases.
Antó JM, Sunyer J, Newman Taylor AJ, 1996, Comparison of soybean epidemic asthma and occupational asthma., Thorax, Vol: 51, Pages: 743-749, ISSN: 0040-6376
Gordon S, Tee RD, Newman Taylor AJ, 1996, Analysis of the allergenic composition of rat dust., Clin Exp Allergy, Vol: 26, Pages: 533-541, ISSN: 0954-7894
BACKGROUND: Allergy to rats is an important occupational health problem. The allergens of rat urine have been well defined but those in rat room dust, a potentially important source of inhalant exposure, have not. OBJECTIVE: To describe the allergens present in rat room dust and to identify a suitable marker protein which may be used to quantify airborne rat allergen. METHODS: Dust collected from the air-conditioning system (bulk dust, 'bd') and with an air sampler (airborne dust, 'ad') were analysed by radioallergosorbent test (RAST) inhibition, immunoblotting and immunoblot inhibition techniques and comparisons made with hair and urine extracts prepared from adult male Wistar rats. RESULTS: Extensive crossreactivity was found between the extracts by RAST inhibition under different experimental conditions. Dust was more potent as an inhibitor than other extracts. The immunoblotting patterns of both dusts were similar although 'ad' contained an allergen at 29 kDa not found in 'bd'. Forty-two sera from rat allergic subjects were used to identify 18 allergens in 'bd'. Three 'major' allergens were found; 100% of subjects had immunoglobulin (Ig)E to a 44 kDa allergen and 74% and 88% of subjects had IgE with bound to the 20.5 and 17 kDa allergens respectively. Immunoblot inhibition experiments identified the 17 kDa dust allergen as alpha 2u-globulin (Rat nI). CONCLUSIONS: Rat dust is a complex allergenic source. The 17 kDa dust allergen has immunological identity with Rat nI and is a suitable marker protein for the quantitation of airborne rat allergen.
Nieuwenhuijsen MJ, Gordon S, Harris JM, et al., 1995, Variation in rat urinary aeroallergen levels explained by differences in site, task and exposure group, ANNALS OF OCCUPATIONAL HYGIENE, Vol: 39, Pages: 819-825, ISSN: 0003-4878
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- Citations: 26
Hayes JP, Newman Taylor AJ, 1995, In vivo models of occupational asthma due to low molecular weight chemicals., Occup Environ Med, Vol: 52, Pages: 539-543, ISSN: 1351-0711
The aim was to review the development of in vivo models of asthma due to low molecular weight chemicals, in particular, those aspects that may be important to the understanding of occupational asthma in humans.
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