Imperial College London
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DrAlexiNott

Faculty of Medicine, Department of Brain Sciences

Lecturer in Neurogenomics, UK DRI Group Leader
 
 
 
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Contact

 

a.nott Website

 
 
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Location

 

Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Breuss:2020:10.1101/2020.08.10.244814,
author = {Breuss, MW and Yang, X and Antaki, D and Schlachetzki, JCM and Lana, AJ and Xu, X and Chai, G and Stanley, V and Song, Q and Newmeyer, TF and Nguyen, A and Cao, B and Nott, A and McEvoy-Venneri, J and Pasillas, MP and Nahas, S and Van, Der Kraan L and Ding, Y and Glass, CK and Gleeson, JG},
doi = {10.1101/2020.08.10.244814},
title = {Somatic mosaicism in the mature brain reveals clonal cellular distributions during cortical development},
url = {http://dx.doi.org/10.1101/2020.08.10.244814},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>The structure of the human neocortex underlies species-specific features and is a reflection of intricate developmental programs. Here we analyzed neocortical cellular lineages through a comprehensive assessment of brain somatic mosaicism—which acts as a neutral recorder of lineage history. We employed deep whole genome and variant sequencing in a single <jats:italic>postmortem</jats:italic> neurotypical human brain across 25 anatomic regions and three distinct modalities: bulk geographies, sorted cell types, and single nuclei. We identified 259 mosaic variants, revealing remarkable differences in localization, clonal abundance, cell type specificity, and clade distribution. We identified a set of hierarchical cellular diffusion barriers, whereby the left-right axis separation of the neocortex occurs prior to anterior-posterior and dorsal-ventral axis separation. We also found that stochastic distribution is a driver of clonal dispersion, and that rules regarding cellular lineages and anatomical boundaries are often ignored. Our data provides a comprehensive analysis of brain somatic mosaicism across the human cerebral cortex, deconvolving clonal distributions and migration patterns in the human embryo.</jats:p><jats:sec><jats:title>One Sentence Summary</jats:title><jats:p>Comprehensive evaluation of brain somatic mosaicism in the adult human identifies rules governing cellular distribution during embryogenesis.</jats:p></jats:sec>
AU  - Breuss,MW
AU  - Yang,X
AU  - Antaki,D
AU  - Schlachetzki,JCM
AU  - Lana,AJ
AU  - Xu,X
AU  - Chai,G
AU  - Stanley,V
AU  - Song,Q
AU  - Newmeyer,TF
AU  - Nguyen,A
AU  - Cao,B
AU  - Nott,A
AU  - McEvoy-Venneri,J
AU  - Pasillas,MP
AU  - Nahas,S
AU  - Van,Der Kraan L
AU  - Ding,Y
AU  - Glass,CK
AU  - Gleeson,JG
DO  - 10.1101/2020.08.10.244814
PY  - 2020///
TI  - Somatic mosaicism in the mature brain reveals clonal cellular distributions during cortical development
UR  - http://dx.doi.org/10.1101/2020.08.10.244814
ER  -
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