22 results found
Pinna A, Cali E, Kerherve G, et al., 2020, Fulleropyrrolidine-functionalized ceria nanoparticles as a tethered dual nanosystem with improved antioxidant properties, NANOSCALE ADVANCES, Vol: 2, Pages: 2387-2396, ISSN: 2516-0230
Mohammed AA, Pinna A, Li S, et al., 2020, Auto-catalytic redox polymerisation using nanoceria and glucose oxidase for double network hydrogels, Journal of Materials Chemistry B, Vol: 8, Pages: 2834-2844, ISSN: 2050-750X
A novel auto-catalytic reaction, a combination of naturally occurring enzyme glucose oxidase (GOx) and amine-functionalised cerium oxide nanoparticles (nanoceria), was employed for open vessel free radical polymerisation of double network hydrogels (DNHGs). The nanoceria also incorporated into the gels to enhance mechanical strength. GOx reduces atmospheric O2 to H2O2, causing a cyclic change of cerium ion states, resulting in propagating free radicals in the carbon group in the amino functionalised nanoceria surface. We synthesised novel nanocomposite DNHGs by grafting polymers onto amine-functionalised nanoceria (ANC), with poly(2-acrylamido-2-methylpropanesulfonic acid), PAMPS, and polyacrylamide (PAAm) in the first and second networks respectively. The graft polymerisation was initiated using the alternating cerium states on the ANC. GOx held two major roles within the reaction: to provide an oxygen free system, without any other form of degassing, and to provide cyclical cerium ion states between Ce4+ and Ce3+, creating new free radicals for polymerisation. Polymer conversion using ANC as the sole initiator in the presence of GOx resulted in 83% conversion for PAMPS and 64% PAAm. Polymers degassed only with argon resulted in less than 55% conversion for both PAAm and PAMPS, proving that the addition of GOx enhanced the reaction. The new gels (1.76 MPa) showed an order of magnitude improvement in mechanical properties compared to DNHG made without ANC/GOx (0.10 MPa).
Ravanbakhsh M, Labbaf S, Karimzadeh F, et al., 2019, Mesoporous bioactive glasses for the combined application of osteosarcoma treatment and bone regeneration, MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, Vol: 104, ISSN: 0928-4931
Falchi L, Galleri G, Dore GM, et al., 2018, Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 degrees C for 96 hours, Reproductive Biology and Endocrinology, Vol: 16, ISSN: 1477-7827
BackgroundCerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2 NPs for 96 h on the main structural and kinematic parameters of spermatozoa.MethodsThe ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender. Samples were exposed to increasing doses of CeO2 NPs (0, 44 and 220 μg/mL) and stored at 4 °C for 96 h. Analyses of kinematic parameters (computer assisted sperm analysis, CASA), integrity of membranes (PI/PSA staining), ROS production (H2DCFDA staining) and DNA damage (sperm chromatin structure assay with acridine orange, SCSA) were performed every 24 h (0, 24, 48, 72 and 96 h of incubation). The experiment was carried out in 6 replicates. Data were analysed by repeated measures ANOVA with Bonferroni’s as post hoc test. When the assumption of normality was not met (ROS), non-parametric Kruskal-Wallis rank test was carried out.ResultsExposure of ram spermatozoa to increasing doses of CeO2 NPs had a beneficial effect on the main motility parameters from 48 h of incubation onward. Velocity of sperm cells was enhanced in the groups exposed to CeO2 NPs compared to the control. Incubation with NPs had beneficial effects on the integrity of plasma membranes of spermatozoa, with higher percentage of damaged cells in the control group compared to the exposed ones. Production of ROS was not affected by exposure to NPs and its levels rose at 96 h of incubation. The integrity of DNA remained stable throughout the 96 h of storage regardless of co-incubation with NPs.ConclusionsWe reported beneficial effects of CeO2 NPs on kinematic and morphologic parameters of ram semen, such as motility and membrane integrity following 96 h of exposure. Furthermore, we also proved no gen
Nommeots-Nomm A, Labbaf S, Devlin A, et al., 2017, Highly degradable porous melt-derived bioactive glass foam scaffolds for bone regeneration, Acta Biomaterialia, Vol: 57, Pages: 449-461, ISSN: 1878-7568
A challenge in using bioactive melt-derived glass in bone regeneration is to produce scaffolds with interconnected pores while maintaining the amorphous nature of the glass and its associated bioactivity. Here we introduce a method for creating porous melt-derived bioactive glass foam scaffolds with low silica content and report in vitro and preliminary in vivo data. The gel-cast foaming process was adapted, employing temperature controlled gelation of gelatin, rather than the in situ acrylic polymerisation used previously. To form a 3D construct from melt derived glasses, particles must be fused via thermal processing, termed sintering. The original Bioglass® 45S5 composition crystallises upon sintering, altering its bioactivity, due to the temperature difference between the glass transition temperature and the crystallisation onset being small. Here, we optimised and compared scaffolds from three glass compositions, ICIE16, PSrBG and 13–93, which were selected due to their widened sintering windows. Amorphous scaffolds with modal pore interconnect diameters between 100–150 µm and porosities of 75% had compressive strengths of 3.4 ± 0.3 MPa, 8.4 ± 0.8 MPa and 15.3 ± 1.8 MPa, for ICIE16, PSrBG and 13–93 respectively. These porosities and compressive strength values are within the range of cancellous bone, and greater than previously reported foamed scaffolds. Dental pulp stem cells attached to the scaffold surfaces during in vitro culture and were viable. In vivo, the scaffolds were found to regenerate bone in a rabbit model according to X-ray micro tomography imaging.
Ariu F, Bogliolo L, Pinna A, et al., 2017, Cerium oxide nanoparticles (CeO2 NPs) improve the developmental competence of in vitro-matured prepubertal ovine oocytes, REPRODUCTION FERTILITY AND DEVELOPMENT, Vol: 29, Pages: 1046-1056, ISSN: 1031-3613
Jiang Y, Carboni D, Pinna A, et al., 2016, Hard X-rays for processing hybrid organic-inorganic thick films., Journal of Synchrotron Radiation, Vol: 23, Pages: 267-273, ISSN: 1600-5775
Hard X-rays, deriving from a synchrotron light source, have been used as an effective tool for processing hybrid organic-inorganic films and thick coatings up to several micrometres. These coatings could be directly modified, in terms of composition and properties, by controlled exposure to X-rays. The physico-chemical properties of the coatings, such as hardness, refractive index and fluorescence, can be properly tuned using the interaction of hard X-rays with the sol-gel hybrid films. The changes in the microstructure have been correlated especially with the modification of the optical and the mechanical properties. A relationship between the degradation rate of the organic groups and the rise of fluorescence from the hybrid material has been observed; nanoindentation analysis of the coatings as a function of the X-ray doses has shown a not linear dependence between thickness and film hardness.
Falchi L, Bogliolo L, Galleri G, et al., 2015, Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure, Theriogenology, Vol: 85, Pages: 1274-1281.e3, ISSN: 0093-691X
The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO2 NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. Spermatozoa were incubated with 0, 22, 44, and 220 μg/mL of CeO2 NPs at 4 °C and submitted at 0, 2, and 24 hours to the following analyses: (1) intracellular uptake of CeO2 NPs by the spermatozoa; (2) kinematic parameters; (3) acrosome and membrane integrity; (4) integrity of DNA; (5) mitochondrial activity; (6) ROS production. The results indicated that the exposure of spermatozoa to increasing doses of nanoceria was well tolerated. No intracellular uptake of NPs by the cells was observed and both kinematic parameters and status of the membranes were not affected by the incubation with NPs (P > 0.05). Moreover, no influence on the redox status of spermatozoa and on the levels of fragmentation of DNA was reported among groups at any time (P > 0.05). The data collected provide new information about the impact of CeO2 NPs on the male gamete in large animal model and could open future perspectives about their biomedical use in the assisted reproductive techniques.
Carboni D, Pinna A, Amenitsch H, et al., 2015, Getting order in mesostructured thin films, from pore organization to crystalline walls, the case of 3-glycidoxypropyltrimethoxysilane, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 10679-10686, ISSN: 1463-9076
Pinna A, Malfatti L, Galleri G, et al., 2015, Ceria nanoparticles for the treatment of Parkinson-like diseases induced by chronic manganese intoxication, RSC ADVANCES, Vol: 5, Pages: 20432-20439
Malfatti L, Pinna A, Enzo S, et al., 2015, Tuning the phase transition of ZnO thin films through lithography: an integrated bottom-up and top-down processing, JOURNAL OF SYNCHROTRON RADIATION, Vol: 22, Pages: 165-171, ISSN: 0909-0495
Innocenzi P, Malfatti L, Lasio B, et al., 2014, Sol-gel chemistry for graphene-silica nanocomposite films, NEW JOURNAL OF CHEMISTRY, Vol: 38, Pages: 3777-3782, ISSN: 1144-0546
Falcaro P, Trinchi A, Doherty C, et al., 2014, 3D Spatially Controlled Chemical Functionalization on Alumina Membranes, SCIENCE OF ADVANCED MATERIALS, Vol: 6, Pages: 1520-1524, ISSN: 1947-2935
Pinna A, Lasio B, Carboni D, et al., 2014, Engineering the surface of hybrid organic-inorganic films with orthogonal grafting of oxide nanoparticles, JOURNAL OF NANOPARTICLE RESEARCH, Vol: 16, ISSN: 1388-0764
Carboni D, Marongiu D, Rassu P, et al., 2014, Enhanced Photocatalytic Activity in Low-Temperature Processed Titania Mesoporous Films, JOURNAL OF PHYSICAL CHEMISTRY C, Vol: 118, Pages: 12000-12009, ISSN: 1932-7447
Malfatti L, Falcaro P, Pinna A, et al., 2014, Exfoliated Graphene into Highly Ordered Mesoporous Titania Films: Highly Performing Nanocomposites from Integrated Processing, ACS APPLIED MATERIALS & INTERFACES, Vol: 6, Pages: 795-802, ISSN: 1944-8244
Carboni D, Pinna A, Malfatti L, et al., 2014, Smart tailoring of the surface chemistry in GPTMS hybrid organic-inorganic films, NEW JOURNAL OF CHEMISTRY, Vol: 38, Pages: 1635-1640, ISSN: 1144-0546
Pinna A, Lasio B, Piccinini M, et al., 2013, Combining Top-Down and Bottom-Up Routes for Fabrication of Mesoporous Titania Films Containing Ceria Nanoparticles for Free Radical Scavenging, ACS APPLIED MATERIALS & INTERFACES, Vol: 5, Pages: 3168-3175, ISSN: 1944-8244
Carboni D, Malfatti L, Pinna A, et al., 2013, Molecularly imprinted La-doped mesoporous titania films with hydrolytic properties toward organophosphate pesticides, NEW JOURNAL OF CHEMISTRY, Vol: 37, Pages: 2995-3002, ISSN: 1144-0546
Pinna A, Figus C, Lasio B, et al., 2012, Release of Ceria Nanoparticles Grafted on Hybrid Organic-Inorganic Films for Biomedical Application, ACS APPLIED MATERIALS & INTERFACES, Vol: 4, Pages: 3916-3922, ISSN: 1944-8244
Pinna A, Malfatti L, Piccinini M, et al., 2012, Hybrid materials with an increased resistance to hard X-rays using fullerenes as radical sponges, JOURNAL OF SYNCHROTRON RADIATION, Vol: 19, Pages: 586-590, ISSN: 0909-0495
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