Imperial College London
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DrAlessandraPinna

Faculty of Engineering, Department of Materials

Honorary Senior Research Fellow
 
 
 
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Contact

 

a.pinna

 
 
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Location

 

2M14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Falchi:2015:10.1071/rdv27n1ab266,
author = {Falchi, L and Bogliolo, L and Galleri, G and Vlachopoulou, G and Murrone, O and Epifani, G and Pinna, A and Innocenzi, P and Ledda, S},
doi = {10.1071/rdv27n1ab266},
journal = {Reproduction, Fertility and Development},
pages = {222--222},
title = {266 BIOCOMPATIBILITY OF NANOCERIA IN RAM SPERM DURING 24 HOURS OF INCUBATION},
url = {http://dx.doi.org/10.1071/rdv27n1ab266},
volume = {27},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:p>In recent years, there has been increasing interest in nanoparticles, especially those widely present in our environment. Several studies have been performed to evaluate their potential toxic effect and their possible use for biomedical applications. Among others, cerium dioxide nanoparticles (nanoceria, CeO2 ENPs) have been recently investigated for their use in biomedicine, based on their potential antioxidant function, due to the presence of oxygen vacancies and redox transformations (Ce4+/Ce3+) occurring at the surface. However, little is known about the potential toxicity of nanoceria in the reproductive system and on gametes, and no information is available with regard to its biocompatibility and potential toxicity on male gametes. The aim of the present study was to investigate effects of increasing doses of CeO2 ENPs on ram spermatozoa during 24 h storage at 4°C, based on assessment of main kinematic parameters, membranes and DNA integrity, ROS production, mitochondrial activity, and CeO2 intracellular uptake. The ejaculates of 3 rams of proven fertility were pooled and incubated with increasing doses of nanoceria (0, 22, 44, and 220 µg mL–1) up to 24 h at 4°C. The experiment was conducted in 4 replicates. At 0, 2, and 24 h of incubation, the 4 groups were submitted to the following analyses: i) main kinematic parameters (total motility and progressive motility) through CASA (computer-assisted sperm analysis); ii) acrosome and membrane integrity (propidium iodide + Pisum sativum agglutinin staining, PI+PSA); iii) flow cytometry for sperm chromatin structure assay (SCSA, acridine orange staining), mitochondrial activity (Mitotracker Orange), and ROS production (H2DCFDA). Moreover, an aliquot of semen from each group in each time step was fixed and processed for transmission electron microscopy to assess intracellular uptake of CeO2 nanoparticles by spermatozoa. Increasing conce
AU  - Falchi,L
AU  - Bogliolo,L
AU  - Galleri,G
AU  - Vlachopoulou,G
AU  - Murrone,O
AU  - Epifani,G
AU  - Pinna,A
AU  - Innocenzi,P
AU  - Ledda,S
DO  - 10.1071/rdv27n1ab266
EP  - 222
PY  - 2015///
SN  - 1031-3613
SP  - 222
TI  - 266 BIOCOMPATIBILITY OF NANOCERIA IN RAM SPERM DURING 24 HOURS OF INCUBATION
T2  - Reproduction, Fertility and Development
UR  - http://dx.doi.org/10.1071/rdv27n1ab266
VL  - 27
ER  -
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