Imperial College London
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DrAlessandraPinna

Faculty of Engineering, Department of Materials

Honorary Senior Research Fellow
 
 
 
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a.pinna

 
 
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Location

 

2M14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Falchi:2018:10.1186/s12958-018-0339-9,
author = {Falchi, L and Galleri, G and Dore, GM and Zedda, MT and Pau, S and Bogliolo, L and Ariu, F and Pinna, A and Nieddu, S and Innocenzi, P and Ledda, S},
doi = {10.1186/s12958-018-0339-9},
journal = {Reproductive Biology and Endocrinology},
title = {Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 degrees C for 96 hours},
url = {http://dx.doi.org/10.1186/s12958-018-0339-9},
volume = {16},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundCerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2 NPs for 96 h on the main structural and kinematic parameters of spermatozoa.MethodsThe ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender. Samples were exposed to increasing doses of CeO2 NPs (0, 44 and 220 μg/mL) and stored at 4 °C for 96 h. Analyses of kinematic parameters (computer assisted sperm analysis, CASA), integrity of membranes (PI/PSA staining), ROS production (H2DCFDA staining) and DNA damage (sperm chromatin structure assay with acridine orange, SCSA) were performed every 24 h (0, 24, 48, 72 and 96 h of incubation). The experiment was carried out in 6 replicates. Data were analysed by repeated measures ANOVA with Bonferroni’s as post hoc test. When the assumption of normality was not met (ROS), non-parametric Kruskal-Wallis rank test was carried out.ResultsExposure of ram spermatozoa to increasing doses of CeO2 NPs had a beneficial effect on the main motility parameters from 48 h of incubation onward. Velocity of sperm cells was enhanced in the groups exposed to CeO2 NPs compared to the control. Incubation with NPs had beneficial effects on the integrity of plasma membranes of spermatozoa, with higher percentage of damaged cells in the control group compared to the exposed ones. Production of ROS was not affected by exposure to NPs and its levels rose at 96 h of incubation. The integrity of DNA remained stable throughout the 96 h of storage regardless of co-incubation with NPs.ConclusionsWe reported beneficial effects of CeO2 NPs on kinematic and morphologic parameters of ram semen, such as motility and membrane integrity following 96 h of exposure. Furthermore, we also proved no gen
AU  - Falchi,L
AU  - Galleri,G
AU  - Dore,GM
AU  - Zedda,MT
AU  - Pau,S
AU  - Bogliolo,L
AU  - Ariu,F
AU  - Pinna,A
AU  - Nieddu,S
AU  - Innocenzi,P
AU  - Ledda,S
DO  - 10.1186/s12958-018-0339-9
PY  - 2018///
SN  - 1477-7827
TI  - Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 degrees C for 96 hours
T2  - Reproductive Biology and Endocrinology
UR  - http://dx.doi.org/10.1186/s12958-018-0339-9
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000427031900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/63158
VL  - 16
ER  -
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