Imperial College London
Alternate

ProfessorAlexandraPorter

Faculty of Engineering, Department of Materials

Professor of Bio-imaging and Analysis
 
 
 
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Contact

 

+44 (0)20 7594 9691a.porter

 
 
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Location

 

B341 Royal School of MinesRoyal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Naruphontjirakul:2017:10.1016/j.actbio.2017.11.008,
author = {Naruphontjirakul, P and Porter, AE and Jones, JR},
doi = {10.1016/j.actbio.2017.11.008},
journal = {Acta Biomaterialia},
pages = {67--80},
title = {In vitro osteogenesis by intracellular uptake of strontium containing bioactive glass nanoparticles.},
url = {http://dx.doi.org/10.1016/j.actbio.2017.11.008},
volume = {66},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Monodispersed strontium containing bioactive glass nanoparticles (Sr-BGNPs) with two compositions were synthesised, through a modified sol-gel Stöber process, wherein silica nanoparticles (SiO2-NPs) were formed prior to incorporation of calcium and strontium, with diameters of 90±10nm. The osteogenic response of a murine preosteoblast cell line, MC3T3-E1, was investigated in vitro for a nanoparticle concentration of 250µg/mL with compositions of 87mol% SiO2, 7mol% CaO, 6mol% SrO and 83mol% SiO2, 3mol% CaO, 14mol% SrO. Dissolution studies in minimum essential media (α-MEM) at pH 7.4 and artificial lysosomal fluid (ALF) at pH 4.5 showed that the particles dissolved and that Sr2+ ions were released from Sr-BGNPs in both environments. Both particle compositions and their ionic dissolution products enhanced the alkaline phosphatase (ALP) activity of the cells and calcium deposition. Immunohistochemistry (IHC) staining of Col1a1, osteocalcin (OSC) and osteopontin (OSP) showed that these proteins were expressed in the MC3T3-E1 cells following three weeks of culture. In the basal condition, the late osteogenic differentiation markers, OSC and OSP, were more overtly expressed by cells cultured with Sr-BGNPs with 14mol% SrO and their ionic release products than in the control condition. Col1a1 expression was only slightly enhanced in the basal condition, but was enhanced further by the osteogenic supplements. These data demonstrate that Sr-BGNPs accelerate mineralisation without osteogenic supplements. Sr-BGNPs were internalised into MC3T3-E1 cells by endocytosis and stimulated osteogenic differentiation of the pre-osteoblast cell line. Sr-BGNPs are likely to be beneficial for bone regeneration and the observed osteogenic effects of these particles can be attributed to their ionic release products. STATEMENT OF SIGNIFICANCE: We report, for the first time, that monodispersed bioactive glass nanoparticles (∼90nm) are internalised into pre
AU  - Naruphontjirakul,P
AU  - Porter,AE
AU  - Jones,JR
DO  - 10.1016/j.actbio.2017.11.008
EP  - 80
PY  - 2017///
SN  - 1742-7061
SP  - 67
TI  - In vitro osteogenesis by intracellular uptake of strontium containing bioactive glass nanoparticles.
T2  - Acta Biomaterialia
UR  - http://dx.doi.org/10.1016/j.actbio.2017.11.008
UR  - http://hdl.handle.net/10044/1/56149
VL  - 66
ER  -
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