Imperial College London
Alternate

ProfessorAlexandraPorter

Faculty of Engineering, Department of Materials

Professor of Bio-imaging and Analysis
 
 
 
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Contact

 

+44 (0)20 7594 9691a.porter

 
 
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Location

 

B341 Royal School of MinesRoyal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kenney:2018:10.1016/j.chemgeo.2018.01.025,
author = {Kenney, JPL and Ellis, T and Nicol, FS and Porter, AE and Weiss, DJ},
doi = {10.1016/j.chemgeo.2018.01.025},
journal = {Chemical Geology},
pages = {61--71},
title = {The effect of bacterial growth phase and culture concentration on U(VI) removal from aqueous solution},
url = {http://dx.doi.org/10.1016/j.chemgeo.2018.01.025},
volume = {482},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacteria play a key role in controlling the mobility of contaminants, such as uranium (U), in the environment. Uranium could be sourced from disposed radioactive waste, derived either from surface disposal trenches for Low Level Waste (LLW) that, because of the waste type and disposal concept, would typically present acidic conditions or from the geological disposal of LLW or Intermediate Level Waste (ILW) that, because of the waste type and the disposal concept, would typically present alkaline conditions. In disposed radioactive waste, there could be variable amounts of cellulosic material. Bacterial cells may be living in a range of different growth phases, depending on the growth conditions and nutrients available at the time any waste-derived U migrated to the cells. A key knowledge gap to date has been the lack of a mechanistic understanding of how bacterial growth phases (exponential, stationary, and death phase) affect the ability of bacteria to remove U(VI) from solution. To address this, we first characterised the cells using potentiometric titrations to detect any differences in proton binding to proton active sites on Pseudomonas putida cells at each growth phase under aerobic conditions, or under anaerobic conditions favourable to U(IV) reoxidation. We then conducted batch U(VI) removal experiments with bacteria at each phase suspended in 1 and 10 ppm U aqueous solutions with the pH adjusted from 2 to 12 as well as with culture concentrations from 0.01 to 10 g/L, to identify the minimal concentration of bacteria in solution necessary to affect U removal. We found that, in death phase, P. putida cells exhibited double the concentration of proton active sites than bacteria grown to exponential and stationary phase. However, we did not see a difference in the extent of U(VI) removal, from a 10 ppm U solution, between the different growth phases as a function of pH (2 to 12). Culture concentration affected U removal between pH 2–8, where U removal dec
AU  - Kenney,JPL
AU  - Ellis,T
AU  - Nicol,FS
AU  - Porter,AE
AU  - Weiss,DJ
DO  - 10.1016/j.chemgeo.2018.01.025
EP  - 71
PY  - 2018///
SN  - 0009-2541
SP  - 61
TI  - The effect of bacterial growth phase and culture concentration on U(VI) removal from aqueous solution
T2  - Chemical Geology
UR  - http://dx.doi.org/10.1016/j.chemgeo.2018.01.025
UR  - http://hdl.handle.net/10044/1/57913
VL  - 482
ER  -
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