Imperial College London

DrAtulPurohit

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Visiting Reader
 
 
 
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Contact

 

+44 (0)20 3313 4287a.purohit Website

 
 
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Location

 

6N2BCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

218 results found

Ganeshapillai D, Woo LWL, Thomas MP, Purohit A, Potter BVLet al., 2018, C-3- and C-4-Substituted Bicyclic Coumarin Sulfamates as Potent Steroid Sulfatase Inhibitors, ACS OMEGA, Vol: 3, Pages: 10748-10772, ISSN: 2470-1343

Synthetic routes to potent bicyclic nonsteroidal sulfamate-based active-site-directed inhibitors of the enzyme steroid sulfatase (STS), an emerging target in the treatment of postmenopausal hormone-dependent diseases, including breast cancer, are described. Sulfamate analogs 9–27 and 28–46 of the core in vivo active two-ring coumarin template, modified at the 4- and 3-positions, respectively, were synthesized to expand structure–activity relationships. α-Alkylacetoacetates were used to synthesize coumarin sulfamate derivatives with 3-position modifications, and the bicyclic ring of other parent coumarins was primarily constructed via the Pechmann synthesis of hydroxyl coumarins. Compounds were examined for STS inhibition in intact MCF-7 breast cancer cells and in placental microsomes. Low nanomolar potency STS inhibitors were achieved, and some were found to inhibit the enzyme in MCF-7 cells ca. 100–500 more potently than the parent 4-methylcoumarin-7-O-sulfamate 3, with the best compounds close in potency to the tricyclic clinical drug Irosustat. 3-Hexyl-4-methylcoumarin-7-O-sulfamate 29 and 3-benzyl-4-methylcoumarin-7-O-sulfamate 41 were particularly effective inhibitors with IC50 values of 0.68 and 1 nM in intact MCF-7 cells and 8 and 32 nM for placental microsomal STS, respectively. They were docked into the STS active site for comparison with estrone 3-O-sulfamate and Irosustat, showing their sulfamate group close to the catalytic hydrated formylglycine residue and their pendant group lying between the hydrophobic sidechains of L103, F178, and F488. Such highly potent STS inhibitors expand the structure–activity relationship for these coumarin sulfamate-based agents that possess therapeutic potential and may be worthy of further development.

Journal article

Stengel C, Newman SP, Leese MP, Thomas MP, Potter BVL, Reed MJ, Purohit A, Foster PAet al., 2015, The In Vitro and In Vivo Activity of the Microtubule Disruptor STX140 Is Mediated by Hif-1 Alpha and CAIX Expression, ANTICANCER RESEARCH, Vol: 35, Pages: 5249-5261, ISSN: 0250-7005

Journal article

Stengel C, Newman SP, Day JM, Chander SK, Jourdan FL, Leese MP, Ferrandis E, Regis-Lydi S, Potter BVL, Reed MJ, Purohit A, Foster PAet al., 2014, In vivo and in vitro properties of STX2484: a novel non-steroidal anti-cancer compound active in taxane-resistant breast cancer, BRITISH JOURNAL OF CANCER, Vol: 111, Pages: 300-308, ISSN: 0007-0920

Journal article

Elliott KM, Dent J, Stanczyk FZ, Woodley L, Coombes RC, Purohit A, Palmieri Cet al., 2014, Effects of aromatase inhibitors and body mass index on steroid hormone levels in women with early and advanced breast cancer, BRITISH JOURNAL OF SURGERY, Vol: 101, Pages: 939-948, ISSN: 0007-1323

Journal article

Leese MP, Jourdan FL, Major MR, Dohle W, Thomas MP, Hamel E, Ferrandis E, Mahon MF, Newman SP, Purohit A, Potter BVLet al., 2014, Synthesis, Anti-tubulin and Antiproliferative SAR of Steroidomimetic Dihydroisoquinolinones, CHEMMEDCHEM, Vol: 9, Pages: 798-812, ISSN: 1860-7179

Journal article

Dohle W, Leese MP, Jourdan FL, Major MR, Bai R, Hamel E, Ferrandis E, Kasprzyk PG, Fiore A, Newman SP, Purohit A, Potter BVLet al., 2014, Synthesis, Antitubulin, and Antiproliferative SAR of C3/C1-Substituted Tetrahydroisoquinolines, CHEMMEDCHEM, Vol: 9, Pages: 350-370, ISSN: 1860-7179

Journal article

Meyer-Losic F, Newman SP, Day JM, Reed MJ, Kasprzyk PG, Purohit A, Foster PAet al., 2013, STX140, but Not Paclitaxel, Inhibits Mammary Tumour Initiation and Progression in C3(1)/SV40T/t-Antigen Transgenic Mice, PLOS ONE, Vol: 8, ISSN: 1932-6203

Journal article

Woo LWL, Wood PM, Bubert C, Thomas MP, Purohit A, Potter BVLet al., 2013, Synthesis and Structure-Activity Relationship Studies of Derivatives of the Dual Aromatase-Sulfatase Inhibitor 4-{[(4-Cyanophenyl)(4H-1,2,4-triazol-4-yl)amino]methyl}phenyl sulfamate, CHEMMEDCHEM, Vol: 8, Pages: 779-799, ISSN: 1860-7179

Journal article

Day JM, Foster PA, Tutill HJ, Schmidlin F, Sharland CM, Hargrave JD, Vicker N, Potter BVL, Reed MJ, Purohit Aet al., 2013, STX2171, a 17 beta-hydroxysteroid dehydrogenase type 3 inhibitor, is efficacious in vivo in a novel hormone-dependent prostate cancer model, ENDOCRINE-RELATED CANCER, Vol: 20, Pages: 53-64, ISSN: 1351-0088

Journal article

Woo LWL, Leblond B, Purohit A, Potter BVLet al., 2012, Synthesis and evaluation of analogues of estrone-3-O-sulfamate as potent steroid sulfatase inhibitors, BIOORGANIC & MEDICINAL CHEMISTRY, Vol: 20, Pages: 2506-2519, ISSN: 0968-0896

Journal article

Meyer-Losic F, Fiore A, Carlson M, Lauer J, Roubert P, Bernetiere S, Oligo C, Vignaux L, Gaudry H, Foster PA, Newman SP, Purohit A, Leese MP, Kasprzyk PGet al., 2012, Safety profile and therapeutic potential in breast cancer of STX140, an original tubulin binding agent, CANCER RESEARCH, Vol: 72, ISSN: 0008-5472

Journal article

Purohit A, Foster PA, 2012, Steroid sulfatase inhibitors for estrogen- and androgen-dependent cancers, JOURNAL OF ENDOCRINOLOGY, Vol: 212, Pages: 99-110, ISSN: 0022-0795

Journal article

Wood PM, Woo LWL, Thomas MP, Mahon MF, Purohit A, Potter BVLet al., 2011, Aromatase and Dual Aromatase-Steroid Sulfatase Inhibitors from the Letrozole and Vorozole Templates, CHEMMEDCHEM, Vol: 6, Pages: 1423-1438, ISSN: 1860-7179

Journal article

Jourdan F, Leese MP, Dohle W, Ferrandis E, Newman SP, Chander S, Purohit A, Potter BVLet al., 2011, Structure-Activity Relationships of C-17-Substituted Estratriene-3-O-sulfamates as Anticancer Agents, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 54, Pages: 4863-4879, ISSN: 0022-2623

Journal article

Purohit A, Woo LWL, Potter BVL, 2011, Steroid sulfatase: A pivotal player in estrogen synthesis and metabolism, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 340, Pages: 154-160, ISSN: 0303-7207

Journal article

Woo LWL, Purohit A, Potter BVL, 2011, Development of steroid sulfatase inhibitors, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 340, Pages: 175-185, ISSN: 0303-7207

Journal article

Geisler J, Sasano H, Chen S, Purohit Aet al., 2011, Steroid sulfatase inhibitors: Promising new tools for breast cancer therapy?, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, Vol: 125, Pages: 39-45, ISSN: 0960-0760

Journal article

Stanway SJ, Palmieri C, Stanczyk FZ, Folkerd EJ, Dowsett M, Ward R, Coombes RC, Reed MJ, Purohit Aet al., 2011, Effect of Tamoxifen or Anastrozole on Steroid Sulfatase Activity and Serum Androgen Concentrations in Postmenopausal Women with Breast Cancer, ANTICANCER RESEARCH, Vol: 31, Pages: 1367-1372, ISSN: 0250-7005

Journal article

Woo LWL, Bubert C, Purohit A, Potter BVLet al., 2011, Hybrid Dual Aromatase-Steroid Sulfatase Inhibitors with Exquisite Picomolar Inhibitory Activity, ACS MEDICINAL CHEMISTRY LETTERS, Vol: 2, Pages: 243-247, ISSN: 1948-5875

Journal article

Woo LWL, Ganeshapillai D, Thomas MP, Sutcliffe OB, Malini B, Mahon MF, Purohit A, Potter BVLet al., 2011, Structure-Activity Relationship for the First-in-Class Clinical Steroid Sulfatase Inhibitor Irosustat (STX64, BN83495), ChemMedChem: chemistry enabling drug discovery, Vol: 6, Pages: 2019-2034

Journal article

Wood PM, Woo LWL, Labrosse J-R, Thomas MP, Mahon MF, Chander SK, Purohit A, Reed MJ, Potter BVLet al., 2010, Bicyclic Derivatives of the Potent Dual Aromatase-Steroid Sulfatase Inhibitor 2-Bromo-4-{[(4-cyanophenyl)(4H-1,2,4-triazol-4-yl)amino]methyl}phenylsulfamate: Synthesis, SAR, Crystal Structure, and in vitro and in vivo Activities, CHEMMEDCHEM, Vol: 5, Pages: 1577-1593, ISSN: 1860-7179

Journal article

Day JM, Foster PA, Tutill HJ, Hargrave JD, Schmidlin F, Vicker N, Potter BVL, Reed MJ, Purohit Aet al., 2010, STX2171, a 17 beta-Hydroxysteroid Dehydrogenase Type 3 (17 beta-HSD3) Inhibitor, Is Efficacious In Vivo in a Novel Hormone-Dependent Prostate Cancer Model., 92nd Meeting and Expo of the Endocrine Society (ENDO 2010), Publisher: ENDOCRINE SOC, ISSN: 0163-769X

Conference paper

Day JM, Tutill HJ, Purohit A, 2010, 17 beta-Hydroxysteroid dehydrogenase inhibitors, MINERVA ENDOCRINOLOGICA, Vol: 35, Pages: 87-108, ISSN: 0391-1977

Journal article

Jourdan F, Leese MP, Dohle W, Hamel E, Ferrandis E, Newman SP, Purohit A, Reed MJ, Potter BVLet al., 2010, Synthesis, Antitubulin, and Antiproliferative SAR of Analogues of 2-Methoxyestradiol-3,17-O,O-bis-sulfamate, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 53, Pages: 2942-2951, ISSN: 0022-2623

Journal article

Woo LWL, Jackson T, Putey A, Cozier G, Leonard P, Acharya KR, Chander SK, Purohit A, Reed MJ, Potter BVLet al., 2010, Highly Potent First Examples of Dual Aromatase-Steroid Sulfatase Inhibitors based on a Biphenyl Template, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 53, Pages: 2155-2170, ISSN: 0022-2623

Journal article

Stengel C, Newman SP, Leese MP, Potter BVL, Reed MJ, Purohit Aet al., 2010, Class III beta-tubulin expression and in vitro resistance to microtubule targeting agents, BRITISH JOURNAL OF CANCER, Vol: 102, Pages: 316-324, ISSN: 0007-0920

Journal article

Leese MP, Jourdan F, Kimberley MR, Cozier GE, Thiyagarajan N, Stengel C, Regis-Lydi S, Foster PA, Newman SP, Acharya KR, Ferrandis E, Purohit A, Reed MJ, Potter BVLet al., 2010, Chimeric microtubule disruptors, CHEMICAL COMMUNICATIONS, Vol: 46, Pages: 2907-2909, ISSN: 1359-7345

Journal article

Foster PA, Ho YT, Newman SP, Leese MP, Potter BVL, Reed MJ, Purohit Aet al., 2009, STX140 and STX641 Cause Apoptosis via the Intrinsic Mitochondrial Pathway and Down-regulate Survivin and XIAP Expression in Ovarian and Prostate Cancer Cells, ANTICANCER RESEARCH, Vol: 29, Pages: 3751-3757, ISSN: 0250-7005

Journal article

Stengel C, Newman S, Foster P, Leese M, Ferrandis E, Regis-Lydi S, Potter B, Reed M, Purohit Aet al., 2009, Anti-cancer activity of a new non-steroidal anti-angiogenic microtubule disruptor in breast cancer, CANCER RESEARCH, Vol: 69, ISSN: 0008-5472

Journal article

Stengel C, Newman S, Foster P, Leese M, Ferrandis E, Regis-Lydi S, Potter B, Reed M, Purohit Aet al., 2009, Anti-cancer activity of a new non-steroidal anti-angiogenic microtubule disruptor in breast cancer, CANCER RESEARCH, Vol: 69, ISSN: 0008-5472

Journal article

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