Imperial College London
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DrAileenRowan

Faculty of Medicine, Department of Infectious Disease

Lecturer in Molecular Virology
 
 
 
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Contact

 

+44 (0)20 7594 3916a.rowan

 
 
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Location

 

VD4Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bangham:2015:10.1186/s12977-015-0221-1,
author = {Bangham, CRM and Melamed, A and Laydon, D and Al, Khatib H and Rowan, A and Taylor, G},
doi = {10.1186/s12977-015-0221-1},
journal = {Retrovirology},
title = {HTLV-1 drives vigorous clonal expansion of infected CD8 + T cells in natural infection},
url = {http://dx.doi.org/10.1186/s12977-015-0221-1},
volume = {12},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundHuman T-lymphotropic Virus Type I (HTLV-1) is a retrovirus that persistently infects 5–10 million individuals worldwide and causes disabling or fatal inflammatory and malignant diseases. The majority of the HTLV-1 proviral load is found in CD4 + T cells, and the phenotype of adult T cell leukemia (ATL) is typically CD4 + . HTLV-1 also infects CD8 + cells in vivo, but the relative abundance and clonal composition of the two infected subpopulations have not been studied. We used a high-throughput DNA sequencing protocol to map and quantify HTLV-1 proviral integration sites in separated populations of CD4 + cells, CD8 + cells and unsorted peripheral blood mononuclear cells from 12 HTLV-1-infected individuals.ResultsWe show that the infected CD8 + cells constitute a median of 5 % of the HTLV-1 proviral load. However, HTLV-1-infected CD8 + clones undergo much greater oligoclonal proliferation than the infected CD4 + clones in infected individuals, regardless of disease manifestation. The CD8 + clones are over-represented among the most abundant clones in the blood and are redetected even after several years.ConclusionsWe conclude that although they make up only 5 % of the proviral load, the HTLV-1-infected CD8 + T-cells make a major impact on the clonal composition of HTLV-1-infected cells in the blood. The greater degree of oligoclonal expansion observed in the infected CD8 + T cells, contrasts with the CD4 + phenotype of ATL; cases of CD8 + adult T-cell leukaemia/lymphoma are rare. This work is consistent with growing evidence that oligoclonal expansion of HTLV-1-infected cells is not sufficient for malignant transformation.
AU  - Bangham,CRM
AU  - Melamed,A
AU  - Laydon,D
AU  - Al,Khatib H
AU  - Rowan,A
AU  - Taylor,G
DO  - 10.1186/s12977-015-0221-1
PY  - 2015///
SN  - 1742-4690
TI  - HTLV-1 drives vigorous clonal expansion of infected CD8 + T cells in natural infection
T2  - Retrovirology
UR  - http://dx.doi.org/10.1186/s12977-015-0221-1
UR  - http://hdl.handle.net/10044/1/27652
VL  - 12
ER  -
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