Imperial College London

Professor Bill Rutherford FRS

Faculty of Natural SciencesDepartment of Life Sciences

Chair in Biochemistry of Solar Energy
 
 
 
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Contact

 

+44 (0)20 7594 5329a.rutherford Website

 
 
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Location

 

702Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

253 results found

Rutherford AW, 2008, The water oxidizing enzyme, 15th European Bioenergetic Conference, Publisher: ELSEVIER SCIENCE BV, Pages: S3-S3, ISSN: 0005-2728

CONFERENCE PAPER

Jamin N, Rutherford AW, 2008, Jean-Michel Neumann - Obituary, TRENDS IN BIOCHEMICAL SCIENCES, Vol: 33, Pages: 297-297, ISSN: 0968-0004

JOURNAL ARTICLE

Rutherford AW, Moore TA, 2008, Mimicking photosynthesis, but just the best bits, NATURE, Vol: 453, Pages: 449-449, ISSN: 0028-0836

JOURNAL ARTICLE

Ishida N, Sugiura M, Rappaport F, Lai TL, Rutherford AW, Boussac Aet al., 2008, Biosynthetic exchange of bromide for chloride and strontium for calcium in the photosystem II oxygen-evolving enzymes., J Biol Chem, Vol: 283, Pages: 13330-13340, ISSN: 0021-9258

The active site for water oxidation in photosystem II goes through five sequential oxidation states (S(0) to S(4)) before O(2) is evolved. It consists of a Mn(4)Ca cluster close to a redox-active tyrosine residue (Tyr(Z)). Cl(-) is also required for enzyme activity. To study the role of Ca(2+) and Cl(-) in PSII, these ions were biosynthetically substituted by Sr(2+) and Br(-), respectively, in the thermophilic cyanobacterium Thermosynechococcus elongatus. Irrespective of the combination of the non-native ions used (Ca/Br, Sr/Cl, Sr/Br), the enzyme could be isolated in a state that was fully intact but kinetically limited. The electron transfer steps affected by the exchanges were identified and then investigated by using time-resolved UV-visible absorption spectroscopy, time-resolved O(2) polarography, and thermoluminescence spectroscopy. The effect of the Ca(2+)/Sr(2+) and Cl(-)/Br(-) exchanges was additive, and the magnitude of the effect varied in the following order: Ca/Cl < Ca/Br < Sr/Cl < Sr/Br. In all cases, the rate of O(2) release was similar to that of the S(3)Tyr(Z)(.) to S(0)Tyr(Z) transition, with the slowest kinetics (i.e. the Sr/Br enzyme) being approximately 6-7 slower than in the native Ca/Cl enzyme. This slowdown in the kinetics was reflected in a decrease in the free energy level of the S(3) state as manifest by thermoluminescence. These observations indicate that Cl(-) is involved in the water oxidation mechanism. The possibility that Cl(-) is close to the active site is discussed in terms of recent structural models.

JOURNAL ARTICLE

Boussac A, Sugiura M, Lai T-L, Rutherford AWet al., 2008, Low-temperature photochemistry in photosystem II from Thermosynechococcus elongatus induced by visible and near-infrared light, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 363, Pages: 1203-1210, ISSN: 0962-8436

JOURNAL ARTICLE

Batista V, Klimov VV, Rutherford AW, Dau H, Hillier W, Barber J, Styring Set al., 2008, Discussion, Philosophical Transactions of the Royal Society B: Biological Sciences, Vol: 363, ISSN: 0962-8436

JOURNAL ARTICLE

Barber J, Rutherford AW, 2008, Introduction, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 363, Pages: 1125-1128, ISSN: 0962-8436

JOURNAL ARTICLE

Herrero C, Lassalle-Kaiser B, Leibl W, Rutherford AW, Aukauloo Aet al., 2008, Artificial systems related to light driven electron transfer processes in PSII, COORDINATION CHEMISTRY REVIEWS, Vol: 252, Pages: 456-468, ISSN: 0010-8545

JOURNAL ARTICLE

Murray JW, Maghlaoui K, Kargul J, Ishida N, Lai T-L, Rutherford AW, Sugiura M, Boussac A, Barber Jet al., 2008, X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II, ENERGY & ENVIRONMENTAL SCIENCE, Vol: 1, Pages: 161-166, ISSN: 1754-5692

JOURNAL ARTICLE

Junge W, Rutherford AW, 2007, Horst Tobias Witt (1922-2007) - Obituary, NATURE, Vol: 448, Pages: 425-425, ISSN: 0028-0836

JOURNAL ARTICLE

Kargul J, Maghlaoui K, Murray J, Deak Z, Boussac A, Rutherford AW, Vass I, Barber Jet al., 2007, Purification, crystallisation and X-ray diffraction analyses of the T elongatus PSII dimer with strontium replacing calcium in the oxygen-evolving complex, BBA Bioenergetics, Vol: 1767

JOURNAL ARTICLE

Aukauloo A, Leibl W, Rutherford AW, 2007, Water photolysis by molecular biomimetics, ACTUALITE CHIMIQUE, Pages: 42-49, ISSN: 0151-9093

JOURNAL ARTICLE

Fufezan C, Gross CM, Sjodin M, Rutherford AW, Krieger-Liszkay A, Kirilovsky Det al., 2007, Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 282, Pages: 12492-12502, ISSN: 0021-9258

JOURNAL ARTICLE

Fufezan C, Zhang CX, Krieger-Liszkay A, Rutherford AWet al., 2005, Secondary quinone in photosystem II of Thermosynechococcus elongatus: Semiquinone-iron EPR signals and temperature dependence of electron transfer, BIOCHEMISTRY, Vol: 44, Pages: 12780-12789, ISSN: 0006-2960

JOURNAL ARTICLE

Boussac A, Sugiura M, Kirilovsky D, Rutherford AWet al., 2005, Near-infrared-induced transitions in the manganese cluster of photosystem II: Action spectra for the S-2 and S-3 redox states, PLANT AND CELL PHYSIOLOGY, Vol: 46, Pages: 837-842, ISSN: 0032-0781

JOURNAL ARTICLE

Fufezan C, Drepper F, Juhnke HD, Lancaster CRD, Un S, Rutherford AW, Krieger-Liszkay Aet al., 2005, Herbicide-induced changes in charge recombination and redox potential of Q(A) in the T4 mutant of Blastochloris viridis, BIOCHEMISTRY, Vol: 44, Pages: 5931-5939, ISSN: 0006-2960

JOURNAL ARTICLE

Sugiura M, Rappaport F, Brettel K, Noguchi T, Rutherford AW, Boussac Aet al., 2005, Site-directed mutagenesis of Thermosynechococcus elongatus photosystem II: the O-2 evolving enzyme lacking the redox active tyrosine D, 46th Annual Meeting of the Japanese-Society-of-Plant-Physiologists, Publisher: OXFORD UNIV PRESS, Pages: S26-S26, ISSN: 0032-0781

CONFERENCE PAPER

Kirilovsky D, Roncel M, Boussac A, Wilson A, Zurita JL, Ducruet JM, Bottin H, Sugiura M, Ortega JM, Rutherford AWet al., 2004, Cytochrome c(550) in the cyanobacterium Thermosynechococcus elongatus - Study of redox mutants, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 279, Pages: 52869-52880, ISSN: 0021-9258

JOURNAL ARTICLE

Zhang CX, Boussac A, Rutherford AW, 2004, Low-temperature electron transfer in photosystem II: A tyrosyl radical and semiquinone charge pair, BIOCHEMISTRY, Vol: 43, Pages: 13787-13795, ISSN: 0006-2960

JOURNAL ARTICLE

Sugiura M, Rappaport F, Brettel K, Noguchi T, Rutherford AW, Boussac Aet al., 2004, Site-directed mutagenesis of the Thermosynechococcus elongatus photosystem II: The O-2-evolving enzyme lacking the redox-active tyrosine D, BIOCHEMISTRY, Vol: 43, Pages: 13549-13563, ISSN: 0006-2960

JOURNAL ARTICLE

Pospisil P, Arato A, Krieger-Liszkay A, Rutherford AWet al., 2004, Hydroxyl radical generation by photosystem II, BIOCHEMISTRY, Vol: 43, Pages: 6783-6792, ISSN: 0006-2960

JOURNAL ARTICLE

Boussac A, Rappaport F, Carrier P, Verbavatz JM, Gobin R, Kirilovsky D, Rutherford AW, Sugiura Met al., 2004, Biosynthetic Ca2+/Sr2+ exchange in the photosystem II oxygen-evolving enzyme of Thermosynechococcus elongatus, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 279, Pages: 22809-22819, ISSN: 0021-9258

JOURNAL ARTICLE

Rutherford AW, Boussac A, Faller P, 2004, The stable tyrosyl radical in Photosystem II: why D?, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Vol: 1655, Pages: 222-230, ISSN: 0005-2728

JOURNAL ARTICLE

Rutherford AW, Boussac A, 2004, Water photolysis in biology, SCIENCE, Vol: 303, Pages: 1782-1784, ISSN: 0036-8075

JOURNAL ARTICLE

Faller P, Goussias C, Rutherford AW, Un Set al., 2003, Resolving intermediates in biological proton-coupled electron transfer: A tyrosyl radical prior to proton movement, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 100, Pages: 8732-8735, ISSN: 0027-8424

JOURNAL ARTICLE

Rutherford AW, Faller P, 2003, Photosystem II: evolutionary perspectives, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 358, Pages: 245-253, ISSN: 0962-8436

JOURNAL ARTICLE

Fufezan C, Rutherford AW, Krieger-Liszkay A, 2002, Singlet oxygen production in herbicide-treated photosystem II, FEBS LETTERS, Vol: 532, Pages: 407-410, ISSN: 0014-5793

JOURNAL ARTICLE

Goussias C, Boussac A, Rutherford AW, 2002, Photosystem II and photosynthetic oxidation of water: an overview, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 357, Pages: 1369-1381, ISSN: 0962-8436

JOURNAL ARTICLE

Faller P, Rutherford AW, Debus RJ, 2002, Tyrosine D oxidation at cryogenic temperature in photosystem II, BIOCHEMISTRY, Vol: 41, Pages: 12914-12920, ISSN: 0006-2960

JOURNAL ARTICLE

Fuhs M, Schnegg A, Prisner T, Kohne I, Hanley J, Rutherford AW, Mobius Ket al., 2002, Orientation selection in photosynthetic PS I multilayers: structural investigation of the charge separated state P (+.)(700) A(1)(-.) by high-field/high-frequency time-resolved EPR at 3.4 T/95 GHz, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Vol: 1556, Pages: 81-88, ISSN: 0005-2728

JOURNAL ARTICLE

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