Imperial College London
Alternate

Professor Bill Rutherford FRS

Faculty of Natural Sciences, Department of Life Sciences

Chair in Biochemistry of Solar Energy
 
 
 
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Contact

 

+44 (0)20 7594 5329a.rutherford Website

 
 
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Location

 

702Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Viola:2022:10.7554/elife.79890,
author = {Viola, S and Roseby, W and Santabarbara, S and Nürnberg, D and Assunção, R and Dau, H and Sellés, J and Boussac, A and Fantuzzi, A and Rutherford, A},
doi = {10.7554/elife.79890},
journal = {eLife},
title = {Impact of energy limitations on function and resilience in long-wavelength photosystem II},
url = {http://dx.doi.org/10.7554/elife.79890},
volume = {11},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Photosystem II (PSII) uses the energy from red light to split water and reduce quinone, an energy-demanding process based on chlorophyll a (Chl-a) photochemistry. Two types of cyanobacterial PSII can use chlorophyll d (Chl-d) and chlorophyll f (Chl-f) to perform the same reactions using lower energy, far-red light. PSII from Acaryochloris marina has Chl-d replacing all but one of its 35 Chl-a, while PSII from Chroococcidiopsis thermalis, a facultative far-red species, has just 4 Chl-f and 1 Chl-d and 30 Chl-a. From bioenergetic considerations, the far-red PSII were predicted to lose photochemical efficiency and/or resilience to photodamage. Here, we compare enzyme turnover efficiency, forward electron transfer, back-reactions and photodamage in Chl-f-PSII, Chl-d-PSII and Chl-a-PSII. We show that: i) all types of PSII have a comparable efficiency in enzyme turnover; ii) the modified energy gaps on the acceptor side of Chl-d-PSII favour recombination via PD1+Phe- repopulation, leading to increased singlet oxygen production and greater sensitivity to high-light damage compared to Chl-a-PSII and Chl-f-PSII; iii) the acceptor-side energy gaps in Chl-f-PSII are tuned to avoid harmful back reactions, favouring resilience to photodamage over efficiency of light usage. The results are explained by the differences in the redox tuning of the electron transfer cofactors Phe and QA and in the number and layout of the chlorophylls that share the excitation energy with the primary electron donor. PSII has adapted to lower energy in two distinct ways, each appropriate for its specific environment but with different functional penalties.
AU  - Viola,S
AU  - Roseby,W
AU  - Santabarbara,S
AU  - Nürnberg,D
AU  - Assunção,R
AU  - Dau,H
AU  - Sellés,J
AU  - Boussac,A
AU  - Fantuzzi,A
AU  - Rutherford,A
DO  - 10.7554/elife.79890
PY  - 2022///
SN  - 2050-084X
TI  - Impact of energy limitations on function and resilience in long-wavelength photosystem II
T2  - eLife
UR  - http://dx.doi.org/10.7554/elife.79890
UR  - https://doi.org/10.7554/eLife.79890
UR  - http://hdl.handle.net/10044/1/98476
VL  - 11
ER  -
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