Publications
71 results found
Shevchuk AI, Novak P, Velazquez MA, et al., 2013, Combined ion conductance and fluorescence confocal microscopy for biological cell membrane transport studies, JOURNAL OF OPTICS, Vol: 15, ISSN: 2040-8978
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- Citations: 5
Miragoli M, Novak P, Ruenraroengsak P, et al., 2013, Functional interaction between charged nanoparticles and cardiac tissue: a new paradigm for cardiac arrhythmia?, NANOMEDICINE, Vol: 8, Pages: 725-737, ISSN: 1743-5889
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- Citations: 40
Klenerman D, Shevchuk A, Novak P, et al., 2013, Imaging the cell surface and its organization down to the level of single molecules, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 368, ISSN: 0962-8436
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- Citations: 15
Takahashi Y, Shevchuk AI, Novak P, et al., 2012, Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: 11540-11545, ISSN: 0027-8424
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- Citations: 169
Shevchuk AI, Novak P, Taylor M, et al., 2012, An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy, JOURNAL OF CELL BIOLOGY, Vol: 197, Pages: 499-508, ISSN: 0021-9525
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- Citations: 65
Babakinejad B, Yasufumi T, Joensson P, et al., 2012, Quantitative Characterization of Local Chemical Delivery through Nanopipette, BIOPHYSICAL JOURNAL, Vol: 102, Pages: 313A-313A, ISSN: 0006-3495
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- Citations: 1
Takahashi Y, Shevchuk AI, Novak P, et al., 2011, Multifunctional nanoprobes for nanoscale chemical imaging and localized chemical delivery at surfaces and interfaces., Angewandte Chemie (International ed. in English), Vol: 50, Pages: 9638-42, ISSN: 1521-3773
Shevchuk AI, 2011, Multifunctional ion conductance microscopy for biophysical studies at nanoscale, 8th EBSA European Biophysics Congress, Publisher: SPRINGER, Pages: 153-153, ISSN: 0175-7571
Shevchuk AI, Novak P, Takahashi Y, et al., 2011, Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe., Nanomedicine (London, England), Vol: 6, Pages: 565-75, ISSN: 1748-6963
Cells naturally operate on the nanoscale level, with molecules combining together to form complex molecular machines, which can work together to enable normal cell function or go wrong as in the case of many diseases. Visualizing these key processes on the nanoscale has been difficult and two main approaches have been used to date; nanometer resolution imaging of fixed cells using electron microscopy, or imaging live cells using optical or fluorescence microscopy, with a resolution of a few hundred nanometers. Scanning probe microscopy has the potential to allow live cells to be imaged at nanoscale resolution and a noncontact method based on the use of a nanopipette probe has been developed over the last 10 years that allows both topographic and functional imaging. The rapid progress in this area of research over the last 4 years is reviewed in this article, which shows that imaging of complex cellular structures and tissues is now possible and that these methods are now sufficiently mature to provide new insights into important diseases.
Miragoli M, Moshkov A, Novak P, et al., 2011, Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells, Journal of the Royal Society Interface, Vol: 8, Pages: 913-925, ISSN: 1742-5662
Cardiovascular diseases are complex pathologies that include alterations of various cell functions at the levels of intact tissue, single cells and subcellular signalling compartments. Conventional techniques to study these processes are extremely divergent and rely on a combination of individual methods, which usually provide spatially and temporally limited information on single parameters of interest. This review describes scanning ion conductance microscopy (SICM) as a novel versatile technique capable of simultaneously reporting various structural and functional parameters at nanometre resolution in living cardiovascular cells at the level of the whole tissue, single cells and at the subcellular level, to investigate the mechanisms of cardiovascular disease. SICM is a multimodal imaging technology that allows concurrent and dynamic analysis of membrane morphology and various functional parameters (cell volume, membrane potentials, cellular contraction, single ion-channel currents and some parameters of intracellular signalling) in intact living cardiovascular cells and tissues with nanometre resolution at different levels of organization (tissue, cellular and subcellular levels). Using this technique, we showed that at the tissue level, cell orientation in the inner and outer aortic arch distinguishes atheroprone and atheroprotected regions. At the cellular level, heart failure leads to a pronounced loss of T-tubules in cardiac myocytes accompanied by a reduction in Z-groove ratio. We also demonstrated the capability of SICM to measure the entire cell volume as an index of cellular hypertrophy. This method can be further combined with fluorescence to simultaneously measure cardiomyocyte contraction and intracellular calcium transients or to map subcellular localization of membrane receptors coupled to cyclic adenosine monophosphate production. The SICM pipette can be used for patch-clamp recordings of membrane potential and single channel currents. In conclusio
Takahashi Y, Shevchuk AI, Novak P, et al., 2010, Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 132, Pages: 10118-10126, ISSN: 0002-7863
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- Citations: 231
Adler J, Shevchuk AI, Novak P, et al., 2010, Plasma membrane topography and interpretation of single-particle tracks., Nature methods, Vol: 7, Pages: 170-1, ISSN: 1548-7105
Novak P, Li C, Shevchuk AI, et al., 2009, Erratum: Corrigendum: Nanoscale live-cell imaging using hopping probe ion conductance microscopy, Nature Methods, Vol: 6, Pages: 935-935, ISSN: 1548-7091
Novak P, Li C, Shevchuk AI, et al., 2009, Nanoscale live-cell imaging using hopping probe ion conductance microscopy, Nature Methods, Vol: 6, Pages: 279-281, ISSN: 1548-7105
We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.
Novak P, Li C, Shevchuk A, et al., 2009, Next Generation SICM Allows Nanoscale Imaging Of Biological Processes In Real-time, Publisher: CELL PRESS, Pages: 374A-374A, ISSN: 0006-3495
Li C, Johnson N, Ostanin V, et al., 2008, High resolution imaging using scanning ion conductance microscopy with improved distance feedback control, PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL, Vol: 18, Pages: 671-677, ISSN: 1002-0071
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- Citations: 24
Shevchuk AI, Hobson P, Lab MJ, et al., 2008, Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy, BIOPHYSICAL JOURNAL, Vol: 94, Pages: 4089-4094, ISSN: 0006-3495
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- Citations: 36
Shevchuk AI, Hobson P, Lab MJ, et al., 2008, Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study, PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, Vol: 456, Pages: 227-235, ISSN: 0031-6768
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- Citations: 35
Dutta AK, Korchev YE, Shevchuk AI, et al., 2008, Spatial distribution of maxi-anion channel on cardiomyocytes detected by smart-patch technique, BIOPHYSICAL JOURNAL, Vol: 94, Pages: 1646-1655, ISSN: 0006-3495
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- Citations: 43
Korchev Y, Sanchez D, Anand P, et al., 2007, "Non-contact" mechanical characterization and stimulation of cells using scanning ion conductance microscopy., 51st Annual Meeting of the Biophysical-Society, Publisher: BIOPHYSICAL SOCIETY, Pages: 33A-33A, ISSN: 0006-3495
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- Citations: 1
Gorelik J, Ali NN, Shevchuk AI, et al., 2006, Functional characterization of embryonic stem cell-derived cardiomyocytes using scanning ion conductance microscopy, TISSUE ENGINEERING, Vol: 12, Pages: 657-664, ISSN: 2152-4947
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- Citations: 21
Rodolfa KT, Bruckbauer A, Zhou DJ, et al., 2006, Nanoscale pipetting for controlled chemistry in small arrayed water droplets using a double-barrel pipet, NANO LETTERS, Vol: 6, Pages: 252-257, ISSN: 1530-6984
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- Citations: 72
Shevchuk AI, Frolenkov GI, Sánchez D, et al., 2006, Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 45, Pages: 2212-2216, ISSN: 1433-7851
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- Citations: 166
Gorelik J, Zhang YJ, Sánchez D, et al., 2005, Aldosterone acts via an ATP autocrine/paracrine system:: The Edelman ATP hypothesis revisited, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 102, Pages: 15000-15005, ISSN: 0027-8424
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- Citations: 47
Zhang YJ, Gorelik J, Sanchez D, et al., 2005, Scanning ion conductance microscopy reveals how a functional renal epithelial monolayer maintains its integrity, KIDNEY INTERNATIONAL, Vol: 68, Pages: 1071-1077, ISSN: 0085-2538
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- Citations: 26
Ying LM, Bruckbauer A, Zhou DJ, et al., 2005, The scanned nanopipette: A new tool for high resolution bioimaging and controlled deposition of biomolecules, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 7, Pages: 2859-2866, ISSN: 1463-9076
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- Citations: 81
Gorelik J, Shevchuk A, de Swiet M, et al., 2004, Comparison of the arrhythmogenic effects of tauro- and glycoconjugates of cholic acid in an <i>in vitro</i> study of rat cardiomyocytes, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 111, Pages: 867-870, ISSN: 1470-0328
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- Citations: 56
Gorelik J, Zhang YJ, Shevchuk AI, et al., 2004, The use of scanning ion conductance microscopy to image A6 cells, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 217, Pages: 101-108, ISSN: 0303-7207
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- Citations: 62
Gorelik J, Vodyanoy I, Shevchuk AI, et al., 2003, Esmolol is antiarrhythmic in doxorubicin-induced arrhythmia in cultured cardiomyocytes - determination by novel rapid cardiomyocyte assay, FEBS LETTERS, Vol: 548, Pages: 74-78, ISSN: 1873-3468
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- Citations: 17
Gorelik J, Shevchuk AI, Frolenkov GI, et al., 2003, Dynamic assembly of surface structures in living cells, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 100, Pages: 5819-5822, ISSN: 0027-8424
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- Citations: 132
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