Imperial College London
Alternate

Dr. Andrew Shevchuk

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 2362a.shevchuk Website

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shevchuk:2016:10.1016/j.bpj.2016.04.017,
author = {Shevchuk, A and Tokar, S and Gopal, S and Sanchez, Alonso JL and Tarasov, A and Vélez-Ortega, AC and Chiappini, C and Rorsman, P and Stevens, MM and Gorelik, J and Frolenkov, GI and Klenerman, D and Korchev, Y},
doi = {10.1016/j.bpj.2016.04.017},
journal = {Biophysical Journal},
pages = {2252--2265},
title = {Angular approach Scanning Ion Conductance Microscopy},
url = {http://dx.doi.org/10.1016/j.bpj.2016.04.017},
volume = {110},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Scanning ion conductance microscopy (SICM) is a super-resolution live imagingtechnique that uses a glass nanopipette as an imaging probe to produce 3D images of cell surface.SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, preciselyposition an imaging nanopipette close to a structure of interest, and use it to obtain ion channelrecordings or locally apply stimuli or drugs. Practical implementations of these SICM advantages,however, are often complicated due to the limitations of currently available SICM systems that“inherited” their design from other scanning probe microscopes in which the scan assembly isplaced right above the specimen. Such arrangement makes the setting of optimal illuminationnecessary for phase contrast or the use of high magnification upright optics difficult. Here wedescribe the designs that allow mounting SICM scanhead on a standard patch-clampmicromanipulator and imaging the sample at an adjustable approach angle. This angle could be asshallow as the approach angle of a patch-clamp pipette between a water immersion objective andthe specimen. Using this angular approach SICM, we obtained topographical images of cells grownon non-transparent nanoneedle arrays, of islets of Langerhans, and of hippocampal neurons under 2upright optical microscope. We also imaged previously inaccessible areas of cells such as the sidesurfaces of the hair cell stereocilia and the intercalated disks of isolated cardiac mocytes, andperformed targeted patch-clamp recordings from the latter. Thus, our new angular approach SICMallows imaging of living cells on non-transparent substrates and a seamless integration with mostpatch-clamp setups on either inverted or upright microscopes, which would facilitate research incell biophysics and physiology.
AU  - Shevchuk,A
AU  - Tokar,S
AU  - Gopal,S
AU  - Sanchez,Alonso JL
AU  - Tarasov,A
AU  - Vélez-Ortega,AC
AU  - Chiappini,C
AU  - Rorsman,P
AU  - Stevens,MM
AU  - Gorelik,J
AU  - Frolenkov,GI
AU  - Klenerman,D
AU  - Korchev,Y
DO  - 10.1016/j.bpj.2016.04.017
EP  - 2265
PY  - 2016///
SN  - 1542-0086
SP  - 2252
TI  - Angular approach Scanning Ion Conductance Microscopy
T2  - Biophysical Journal
UR  - http://dx.doi.org/10.1016/j.bpj.2016.04.017
UR  - http://hdl.handle.net/10044/1/31242
VL  - 110
ER  -
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