Imperial College London
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DrAranSinganayagam

Faculty of Medicine, Department of Infectious Disease

MRC Clinician Scientist Fellow.
 
 
 
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Contact

 

a.singanayagam

 
 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Singanayagam:2019:10.1101/632372,
author = {Singanayagam, A and Loo, S-L and Calderazzo, M and Finney, LJ and Trujillo, Torralbo M-B and Bakhsoliani, E and Girkin, J and Veerati, P and Pathinayake, PS and Nichol, KS and Reid, A and Foottit, J and Johnston, SL and Bartlett, NW and Mallia, P},
doi = {10.1101/632372},
title = {Anti-microbial immunity is impaired in COPD patients with frequent exacerbations},
url = {http://dx.doi.org/10.1101/632372},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>ABSTRACT</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Patients with frequent exacerbations represent a chronic obstructive pulmonary disease (COPD) sub-group requiring better treatment options. The aim of this study was to determine the innate immune mechanisms that underlie susceptibility to frequent exacerbations in COPD.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We measured sputum expression of immune mediators and bacterial loads in samples from patients with COPD at stable state and during virus-associated exacerbations.<jats:italic>Ex vivo</jats:italic>immune responses to rhinovirus infection in differentiated bronchial epithelial cells (BECs) sampled from patients with COPD were additionally evaluated. Patients were stratified as frequent exacerbators (≥2 exacerbations in the preceding year) or infrequent exacerbators (<2 exacerbations in the preceding year) with comparisons made between these groups.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Frequent exacerbators had reduced sputum cell mRNA expression of the anti-viral immune mediators type I and III interferons and reduced interferon-stimulated gene (ISG) expression when clinically stable and during virus-associated exacerbation. RV-induction of interferon and ISGs<jats:italic>ex vivo</jats:italic>was also impaired in differentiated BECs from frequent exacerbators. Frequent exacerbators also had reduced sputum levels of the anti-microbial peptide mannose-binding lectin (MBL)-2 with an associated increase in sputum bacterial loads at 2 weeks following virus-associated exacerbation onset. MBL-2 levels correlated negatively with bacterial loads during exacerbation.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>These data imp
AU  - Singanayagam,A
AU  - Loo,S-L
AU  - Calderazzo,M
AU  - Finney,LJ
AU  - Trujillo,Torralbo M-B
AU  - Bakhsoliani,E
AU  - Girkin,J
AU  - Veerati,P
AU  - Pathinayake,PS
AU  - Nichol,KS
AU  - Reid,A
AU  - Foottit,J
AU  - Johnston,SL
AU  - Bartlett,NW
AU  - Mallia,P
DO  - 10.1101/632372
PY  - 2019///
TI  - Anti-microbial immunity is impaired in COPD patients with frequent exacerbations
UR  - http://dx.doi.org/10.1101/632372
ER  -
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