Imperial College London
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DrAranSinganayagam

Faculty of Medicine, Department of Infectious Disease

MRC Clinician Scientist Fellow.
 
 
 
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Contact

 

a.singanayagam

 
 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pinto:2022:10.1038/s41467-022-29255-y,
author = {Pinto, AL and Rai, RK and Brown, JC and Griffin, P and Edgar, JR and Shah, A and Singanayagam, A and Hogg, C and Barclay, WS and Futter, CE and Burgoyne, T},
doi = {10.1038/s41467-022-29255-y},
journal = {Nature Communications},
pages = {1--14},
title = {Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium},
url = {http://dx.doi.org/10.1038/s41467-022-29255-y},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Ultrastructural studies of SARS-CoV-2 infected cells are crucial to better understand the mechanisms of viral entry and budding within host cells. Here, we examined human airway epithelium infected with three different isolates of SARS-CoV-2 including the B.1.1.7 variant by transmission electron microscopy and tomography. For all isolates, the virus infected ciliated but not goblet epithelial cells. Key SARS-CoV-2 entry molecules, ACE2 and TMPRSS2, were found to be localised to the plasma membrane including microvilli but excluded from cilia. Consistently, extracellular virions were seen associated with microvilli and the apical plasma membrane but rarely with ciliary membranes. Profiles indicative of viral fusion where tomography showed that the viral membrane was continuous with the apical plasma membrane and the nucleocapsids diluted, compared with unfused virus, demonstrate that the plasma membrane is one site of entry where direct fusion releasing the nucleoprotein-encapsidated genome occurs. Intact intracellular virions were found within ciliated cells in compartments with a single membrane bearing S glycoprotein. Tomography showed concentration of nucleocapsids round the periphery of profiles strongly suggestive of viral budding into these compartments and this may explain how virions gain their S glycoprotein containing envelope.
AU  - Pinto,AL
AU  - Rai,RK
AU  - Brown,JC
AU  - Griffin,P
AU  - Edgar,JR
AU  - Shah,A
AU  - Singanayagam,A
AU  - Hogg,C
AU  - Barclay,WS
AU  - Futter,CE
AU  - Burgoyne,T
DO  - 10.1038/s41467-022-29255-y
EP  - 14
PY  - 2022///
SN  - 2041-1723
SP  - 1
TI  - Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-022-29255-y
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000773315800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41467-022-29255-y
UR  - http://hdl.handle.net/10044/1/104716
VL  - 13
ER  -
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