Since its inception, microscopy has revolutionized our vision of Nature, giving insight into a world invisible to the naked eye, while advancing our knowledge and understanding of Life. Alas, the resolution of conventional microscopes has long been limited by the so-called diffraction limit. Thankfully, the last two decades have witnessed the advent of a new range of techniques capable of surpassing the diffraction limit, and the birth of a new field: Super-Resolution Microscopy.
I research super-resolution microscopy, specifically Structured Illumination Microscopy (SIM) in the Rowlands Lab. This technique enables 3D multi-colour live-imaging of biological samples at twice the resolution of standard widefield microscopes. I'm currently developing new methods for increasing speed and throughput in SIM. If you are a biologist or a neuroscientist who would be interested in imaging biological features below 250nm at high speed, feel free to contact me!
Boualam A, Rowlands C, 2021, A method for assessing the spatiotemporal resolution of structured illumination microscopy (SIM), Biomedical Optics Express, Vol:12, ISSN:2156-7085, Pages:790-801
et al., 2019, A PANI supported lipid bilayer that contains NhaA transporter proteins provides a basis for a biomimetic biocapacitor, Chemical Communications, Vol:55, ISSN:1359-7345, Pages:13152-13155
et al., 2017, Performance and stability of chitosan-MWCNTs-laccase biocathode: Effect of MWCNTs surface charges and ionic strength, Journal of Electroanalytical Chemistry, Vol:799, ISSN:1572-6657, Pages:26-33
Boualam A, Rowlands CJ, 2022, Can we see both small and fast? Investigating the speed limits of Structured Illumination Microscopy (SIM), Single Molecule Spectroscopy and Superresolution Imaging XV, SPIE