Imperial College London
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DrAilsaHart

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Practice
 
 
 
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Contact

 

ailsa.hart

 
 
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Location

 

Northwick ParkNorthwick Park and St Marks Site

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Summary

 

Publications

Citation

BibTex format

@article{Bernardo:2012:10.1002/ibd.21893,
author = {Bernardo, D and Mann, ER and Al-Hassi, HO and Clark, SK and McCarthy, NE and Milestone, AN and Cochrane, SA and Hart, AL and Stagg, AJ and Knight, SC},
doi = {10.1002/ibd.21893},
journal = {Inflammatory Bowel Diseases},
pages = {1275--1286},
title = {Human gut-specific homeostatic dendritic cells are generated from blood precursors by the gut microenvironment.},
url = {http://dx.doi.org/10.1002/ibd.21893},
volume = {7},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Dendritic cells (DC) dictate not only the type of T-cell immunity, but also homing patterns of T cells in mice. In humans, we characterized normal human gut DC and tested whether gut-specific homeostatic DC could be generated from blood precursors by factors in the gut microenvironment.METHODS: We characterized the phenotype and function of healthy human gut DC compared with blood and skin DC, and studied whether conditioning of blood DC in the presence of colonic biopsy supernatants (Bx-SN) induced gut-like phenotype and functions.RESULTS: Blood DC mostly expressed both gut and skin homing markers, indicating potential to migrate to both major immune surface organs, and induced multi-homing T cells. However, DC within gut or skin did not demonstrate this multi-homing phenotype, were tissue-specific, and induced tissue-specific T cells. Human gut DC were less stimulatory for allogeneic T cells than their dermal and blood counterparts. Human blood DC cultured in vitro lost homing marker expression. Conditioning of human enriched blood DC with colonic Bx-SN from healthy controls induced a gut-homing phenotype and a homeostatic profile. Moreover, Bx-SN-conditioned DC demonstrated a restricted T-cell stimulatory capacity and preferentially induced gut-specific T cells. Retinoic acid and transforming growth factor beta (TGF-β) mediated the acquisition of the gut-homing and homeostatic properties, respectively, induced by colonic Bx-SN on blood enriched DC.CONCLUSIONS: Tissue-specific factors manipulate immunity via modulating characteristics of DC and may provide tools to generate tissue-specific immunotherapy. (Inflamm Bowel Dis 2011;).
AU  - Bernardo,D
AU  - Mann,ER
AU  - Al-Hassi,HO
AU  - Clark,SK
AU  - McCarthy,NE
AU  - Milestone,AN
AU  - Cochrane,SA
AU  - Hart,AL
AU  - Stagg,AJ
AU  - Knight,SC
DO  - 10.1002/ibd.21893
EP  - 1286
PY  - 2012///
SP  - 1275
TI  - Human gut-specific homeostatic dendritic cells are generated from blood precursors by the gut microenvironment.
T2  - Inflammatory Bowel Diseases
UR  - http://dx.doi.org/10.1002/ibd.21893
VL  - 7
ER  -
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