Publications

BibTex format

@article{Cambridge:2014:10.1371/journal.pone.0107513,
author = {Cambridge, G and Moura, RA and Santos, T and Khawaja, AA and Polido-Pereira, J and Canhao, H and Leandro, MJ and Fonseca, JE},
doi = {10.1371/journal.pone.0107513},
journal = {PLoS One},
pages = {1--9},
title = {Expression of the inherently autoreactive idiotope 9G4 on autoantibodies to citrullinated peptides and on rheumatoid factors in patients with early and established rheumatoid arthritis},
url = {http://dx.doi.org/10.1371/journal.pone.0107513},
volume = {9},
year = {2014}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundThe pre-symptomatic stage of Rheumatoid arthritis (RA) is associated with pro-inflammatory cytokines and autoantibodies. High levels and epitope spread by Rheumatoid factors (RhF) and autoantibodies to citrullinated proteins signify progression towards disease expression. In established RA, the persistence of high autoantibody levels reflects production by both long-lived plasma cells and short-lived plasmablasts. Neither the relative contributions to pathogenesis by autoantibodies from either source, nor the factors responsible for deciding the fate of autoantigen specific ‘parent’ B-cells, is understood. Phenotypic markers identifying subsets of autoreactive B-cells are therefore of interest in understanding the origin and perpetuation of the autoimmune response in RA. One such phenotypic marker is the rat monoclonal antibody, 9G4, which recognises an idiotope on immunoglobuins derived from the inherently autoreactive VH-gene, VH4-34. We therefore investigated whether the 9G4 idiotope was expressed on autoantibodies in patients with RA.Methodology/Principal FindingsSera from 19 patients with established RA and those with <1year history of untreated polyarthritis either resolving into RA (n=42) or non-RA diagnosis (n=31) were included. Autoantibodies to cyclic citrullinated peptides (CCP), RhF and co-expression of the 9G4 idiotope were measured by ELISA. 9G4 recognised a population of anti-CCP antibodies in the majority of sera from patients with established disease and also in samples from patients with early disaese. 9G4+RhF levels were generally lower and not associated with positivity for, or levels of 9G4+CCP.Conclusions/SignificanceThe persistence of 9G4+ immunoglobulins, of any isotype, in serum is rare. We describe here the novel finding of 9G4 expression on anti-CCP antibodies in patients from the earliest symptoms of RA through to established disease. Our results suggest that 9G4 expression on anti-CCP autoantibodies was not d
AU  - Cambridge,G
AU  - Moura,RA
AU  - Santos,T
AU  - Khawaja,AA
AU  - Polido-Pereira,J
AU  - Canhao,H
AU  - Leandro,MJ
AU  - Fonseca,JE
DO  - 10.1371/journal.pone.0107513
EP  - 9
PY  - 2014///
SN  - 1932-6203
SP  - 1
TI  - Expression of the inherently autoreactive idiotope 9G4 on autoantibodies to citrullinated peptides and on rheumatoid factors in patients with early and established rheumatoid arthritis
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0107513
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341774800078&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107513
UR  - http://hdl.handle.net/10044/1/83468
VL  - 9
ER  -

Download

DrAkifKhawaja

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)