Imperial College London
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Dr Alex Ivanov

Faculty of Natural Sciences, Department of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5752alex.ivanov Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

110JMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2023:10.1021/jacs.2c13465,
author = {Wang, X and Thomas, T-M and Ren, R and Zhou, Y and Zhang, P and Li, J and Cai, S and Liu, K and Ivanov, AP and Herrmann, A and Edel, JB},
doi = {10.1021/jacs.2c13465},
journal = {Journal of the American Chemical Society},
pages = {6371--6382},
title = {Nanopore detection using supercharged polypeptide molecular carriers},
url = {http://dx.doi.org/10.1021/jacs.2c13465},
volume = {145},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The analysis at the single-molecule level of proteins and their interactions can provide critical information for understanding biological processes and diseases, particularly for proteins present in biological samples with low copy numbers. Nanopore sensing is an analytical technique that allows label-free detection of single proteins in solution and is ideally suited to applications, such as studying protein-protein interactions, biomarker screening, drug discovery, and even protein sequencing. However, given the current spatiotemporal limitations in protein nanopore sensing, challenges remain in controlling protein translocation through a nanopore and relating protein structures and functions with nanopore readouts. Here, we demonstrate that supercharged unstructured polypeptides (SUPs) can be genetically fused with proteins of interest and used as molecular carriers to facilitate nanopore detection of proteins. We show that cationic SUPs can substantially slow down the translocation of target proteins due to their electrostatic interactions with the nanopore surface. This approach enables the differentiation of individual proteins with different sizes and shapes via characteristic subpeaks in the nanopore current, thus facilitating a viable route to use polypeptide molecular carriers to control molecular transport and as a potential system to study protein-protein interactions at the single-molecule level.
AU  - Wang,X
AU  - Thomas,T-M
AU  - Ren,R
AU  - Zhou,Y
AU  - Zhang,P
AU  - Li,J
AU  - Cai,S
AU  - Liu,K
AU  - Ivanov,AP
AU  - Herrmann,A
AU  - Edel,JB
DO  - 10.1021/jacs.2c13465
EP  - 6382
PY  - 2023///
SN  - 0002-7863
SP  - 6371
TI  - Nanopore detection using supercharged polypeptide molecular carriers
T2  - Journal of the American Chemical Society
UR  - http://dx.doi.org/10.1021/jacs.2c13465
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36897933
UR  - https://pubs.acs.org/doi/10.1021/jacs.2c13465
UR  - http://hdl.handle.net/10044/1/103255
VL  - 145
ER  -
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