Imperial College London
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Dr Alex Thompson

Faculty of Medicine, Department of Surgery & Cancer

Lecturer in Sensing in Cancer
 
 
 
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Contact

 

alex.thompson08 Website

 
 
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Location

 

B411Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Thompson:2014:10.1117/12.2037851,
author = {Thompson, AJ and Tang, T-YD and Herling, TW and Hak, CRC and Mann, S and Knowles, TPJ and Kuimova, MK},
doi = {10.1117/12.2037851},
publisher = {SPIE- Society of Photo-optical Instrumentation Engineers},
title = {Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors},
url = {http://dx.doi.org/10.1117/12.2037851},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - Molecular rotors are fluorophores that have a fluorescence quantum yield that depends upon intermolecular rotation. The fluorescence quantum yield, intensity and lifetime of molecular rotors all vary as functions of viscosity, as high viscosities inhibit intermolecular rotation and cause an increase in the non-radiative decay rate. As such, molecular rotors can be used to probe viscosity on microscopic scales. Here, we apply fluorescence lifetime imaging microscopy (FLIM) to measure the fluorescence lifetimes of three different molecular rotors, in order to determine the microscopic viscosity in two model systems with significant biological interest. First, the constituents of a novel protocell – a model of a prebiotic cell – were studied using the molecular rotors BODIPY C10 and kiton red. Second, amyloid formation was investigated using the molecular rotor Cy3. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
AU  - Thompson,AJ
AU  - Tang,T-YD
AU  - Herling,TW
AU  - Hak,CRC
AU  - Mann,S
AU  - Knowles,TPJ
AU  - Kuimova,MK
DO  - 10.1117/12.2037851
PB  - SPIE- Society of Photo-optical Instrumentation Engineers
PY  - 2014///
SN  - 0277-786X
TI  - Quantitative sensing of microviscosity in protocells and amyloid materials using fluorescence lifetime imaging of molecular rotors
UR  - http://dx.doi.org/10.1117/12.2037851
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336037200028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/31343
ER  -
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